{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T04:35:27Z","timestamp":1759638927904,"version":"3.40.4"},"reference-count":31,"publisher":"Cambridge University Press (CUP)","issue":"11","license":[{"start":{"date-parts":[[2014,6,16]],"date-time":"2014-06-16T00:00:00Z","timestamp":1402876800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Toxoplasma gondii<\/jats:italic>is a widely spread protozoon in humans, mammals and poultry. Regarding the latter, nothing is known yet about the duration of<jats:italic>T. gondii<\/jats:italic>persistence and distribution over a conventional fattening cycle of turkeys and chickens. Twenty-four turkeys and 12 broiler chickens were infected intravenously with 1\u00d710<jats:sup>6<\/jats:sup><jats:italic>T. gondii<\/jats:italic>tachyzoites (strain NED). Serum antibody levels were determined weekly by ELISA (turkeys) or immunofluorescent antibody test (chickens). Turkeys were slaughtered at 4, 8, 12 and 16 weeks post-infection (p.i.), and chickens 5 or 10 weeks p.i. (<jats:italic>n<\/jats:italic>\u00a0=\u00a06 per group). Sixteen different tissue samples per bird were analysed for<jats:italic>T. gondii<\/jats:italic>by PCR. All infected animals showed seroconversion. In turkeys, 15\u00b79% of all samples were tested positive for<jats:italic>T.-gondii<\/jats:italic>-DNA. Among the edible tissues (drumstick, thigh, breast muscle, heart, liver and gizzard) 7\u00b78% tested positive. Among poultry slaughtered after different periods of time after infection no significant differences (<jats:italic>P<\/jats:italic>&gt;0\u00b705) regarding the number of positive samples were observed. Only 4 out of 192 samples (2\u00b71%) from infected chickens contained detectable<jats:italic>T. gondii<\/jats:italic>DNA.The PCR findings suggested that<jats:italic>T. gondii<\/jats:italic>may persist in poultry. Particularly in turkey it was shown that edible tissues stay infected for at least 16 weeks p.i. which indicates a potential risk for consumers of undercooked turkey meat whereas chickens appear less susceptible to<jats:italic>T. gondii<\/jats:italic>infection.<\/jats:p>","DOI":"10.1017\/s003118201400078x","type":"journal-article","created":{"date-parts":[[2014,6,16]],"date-time":"2014-06-16T18:17:20Z","timestamp":1402942640000},"page":"1359-1364","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":17,"title":["Persistence of<i>Toxoplasma gondii<\/i>tissue stages in poultry over a conventional fattening 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