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In the present study, we aimed to produce poly(lactide-co-glycolide) (PLG) microparticles (MPs) containing recombinant SAG2 (rSAG2) to induce improved immunity against <jats:italic>T. gondii<\/jats:italic>. The resulting PLG-encapsulated rSAG2 (PLG-rSAG2) MPs, 2\u00b714\u20133\u00b763\u00a0<jats:italic>\u03bc<\/jats:italic>m in diameter, showed 74\u201380% entrapment efficiency and gradually released antigenic rSAG2 protein (88\u00b73% of the total protein load) for a long 33-day period. Peritoneal immunization with PLG-rSAG2 MPs in BALB\/c mice resulted in not only sustained (10 weeks) lymphocyte proliferation and IFN-<jats:italic>\u03b3<\/jats:italic> production but also an improved protective capacity (87%) against a lethal subcutaneous challenge of 1\u00d710<jats:sup>4<\/jats:sup> live tachyzoites of <jats:italic>T. gondii<\/jats:italic> (RH strain). In conclusion, the sustained release of rSAG2 protein from PLG-rSAG2 MPs extends Th1 cell-mediated immunity (lymphocyte proliferation and IFN-<jats:italic>\u03b3<\/jats:italic> production) and induces improved protection against <jats:italic>T. gondii<\/jats:italic> tachyzoite infection in mice.<\/jats:p>","DOI":"10.1017\/s0031182014000997","type":"journal-article","created":{"date-parts":[[2014,7,18]],"date-time":"2014-07-18T13:47:13Z","timestamp":1405691233000},"page":"1657-1666","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":10,"title":["Sustained release of recombinant surface antigen 2 (rSAG2) from poly(lactide-co-glycolide) microparticles extends protective cell-mediated immunity against <i>Toxoplasma gondii<\/i> in 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