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Fleas were sampled at 12 different time-points throughout the study period (D1\u2013D28) and <jats:italic>A. reconditum<\/jats:italic> was detected by dissection, PCR and histology. Of 2105 fleas fed with infected dog blood, 891 (38\u00b77%) died during the study before being sampled whilst the remaining (<jats:italic>n<\/jats:italic>\u00a0=\u00a01214) were examined for <jats:italic>A. reconditum<\/jats:italic>. Upon dissection, first-stage larvae (L1) were identified after 2 days post infection (D2), second-stage (L2) at D13 and infective third-stage larvae (L3) at D15. Eighteen (30%) of 60 pools of fleas molecularly examined tested positive. Histologically, L2 were detected at D13 in the sub-cuticle region embedded in the back muscle of one female flea. This study provides original data on larval development of <jats:italic>A. reconditum<\/jats:italic> in <jats:italic>C. felis<\/jats:italic> and reports on the usefulness of the artificial feeding system.<\/jats:p>","DOI":"10.1017\/s0031182014001000","type":"journal-article","created":{"date-parts":[[2014,7,28]],"date-time":"2014-07-28T13:47:37Z","timestamp":1406555257000},"page":"1718-1725","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":31,"title":["Development of <i>Acanthocheilonema reconditum<\/i> (Spirurida, Onchocercidae) in the cat flea <i>Ctenocephalides felis<\/i> (Siphonaptera, Pulicidae)"],"prefix":"10.1017","volume":"141","author":[{"given":"ETTORE","family":"NAPOLI","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"EMANUELE","family":"BRIANTI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LUIGI","family":"FALSONE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GABRIELLA","family":"GAGLIO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SABRINA","family":"FOIT","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FRANCESCA","family":"ABRAMO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GIADA","family":"ANNOSCIA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FILIPE","family":"DANTAS-TORRES","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SALVATORE","family":"GIANNETTO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DOMENICO","family":"OTRANTO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2014,7,28]]},"reference":[{"key":"S0031182014001000_ref36","doi-asserted-by":"publisher","DOI":"10.2307\/3281950"},{"key":"S0031182014001000_ref9","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182011002198"},{"key":"S0031182014001000_ref57","first-page":"43","volume-title":"Heartworm 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