{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T19:21:28Z","timestamp":1772306488438,"version":"3.50.1"},"reference-count":50,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2014,8,11]],"date-time":"2014-08-11T00:00:00Z","timestamp":1407715200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>To detect the expression of pro-fibrotic molecules, such as heat shock protein 47 (Hsp47), transforming growth factor-beta 1 (TGF-<jats:italic>\u03b2<\/jats:italic>1) and connective tissue growth factor (CTGF) in liver specimens, and analyse their correlations with the progression of schistosomal hepatic fibrosis, liver biopsy was performed in 42 chronic schistosomiasis (CS) patients, 16 chronic hepatitis B (CHB) patients and five healthy individuals (HI). Immunohistochemistry (IHC) analyses displayed that the expression of Hsp47, TGF-<jats:italic>\u03b2<\/jats:italic>1 and CTGF was increased in CS and CHB patients compared with HI. Using real-time PCR, the mRNA levels of Hsp47, TGF-<jats:italic>\u03b2<\/jats:italic>1 and CTGF were higher in CS patients compared with HI. In CS patients, the mRNA levels of these genes were correlated with the stage of fibrosis, and TGF-<jats:italic>\u03b2<\/jats:italic>1 mRNA expression was associated with the grade of inflammation. Additional analyses indicated that the mRNA levels of Hsp47 and CTGF were highly correlated with liver stiffness value and spleen thickness diameter, both of which represented the severity of fibrosis. In conclusion, the three molecules are involved in the pathogenesis of hepatic fibrosis infected by <jats:italic>Schistosoma japonicum<\/jats:italic>. TGF-<jats:italic>\u03b2<\/jats:italic>1 participates not only in the inflammatory process, but also in the fibrotic process in which Hsp47 and CTGF probably play a key role.<\/jats:p>","DOI":"10.1017\/s0031182014001115","type":"journal-article","created":{"date-parts":[[2014,8,11]],"date-time":"2014-08-11T13:23:53Z","timestamp":1407763433000},"page":"341-351","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":24,"title":["Expression of heat shock protein 47, transforming growth factor-beta 1, and connective tissue growth factor in liver tissue of patients with <i>Schistosoma japonicum<\/i>-induced hepatic fibrosis"],"prefix":"10.1017","volume":"142","author":[{"given":"LAN","family":"LI","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"TING","family":"WU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JIAQUAN","family":"HUANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KE","family":"MA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LEI","family":"XU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HONGWU","family":"WANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"XIANGXUE","family":"FAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RAN","family":"TAO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GUO","family":"AI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"QIN","family":"NING","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2014,8,11]]},"reference":[{"key":"S0031182014001115_ref34","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00044-09"},{"key":"S0031182014001115_ref48","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2010.55"},{"key":"S0031182014001115_ref26","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2893.2001.00314.x"},{"key":"S0031182014001115_ref3","doi-asserted-by":"publisher","DOI":"10.1007\/s004280050086"},{"key":"S0031182014001115_ref6","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199104043241401"},{"key":"S0031182014001115_ref1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0676.2000.020004296.x"},{"key":"S0031182014001115_ref47","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.10.2204"},{"key":"S0031182014001115_ref15","first-page":"185","article-title":"Immunohistochemical localization of transforming growth factor beta-1 and its relationship with collagen expression in advanced liver fibrosis due to biliary atresia","volume":"3","author":"Farrington","year":"2010","journal-title":"Clinical and Experimental Gastroenterology"},{"key":"S0031182014001115_ref35","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762010000400018"},{"key":"S0031182014001115_ref31","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v9.i11.2574"},{"key":"S0031182014001115_ref27","doi-asserted-by":"publisher","DOI":"10.1007\/s004410050594"},{"key":"S0031182014001115_ref25","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00305.2002"},{"key":"S0031182014001115_ref5","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2010.214650"},{"key":"S0031182014001115_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.08.009"},{"key":"S0031182014001115_ref23","first-page":"20","article-title":"Expression of transforming growth factor-beta1 and connective tissue growing factor in schistosomal hepatic fibrosis of mice","volume":"30","author":"Huang","year":"2012","journal-title":"Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi"},{"key":"S0031182014001115_ref17","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2008.03.003"},{"key":"S0031182014001115_ref14","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-012-1509-z"},{"key":"S0031182014001115_ref9","doi-asserted-by":"publisher","DOI":"10.1136\/gut.27.6.734"},{"key":"S0031182014001115_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2013.05.027"},{"key":"S0031182014001115_ref12","doi-asserted-by":"publisher","DOI":"10.1007\/s11596-012-0012-z"},{"key":"S0031182014001115_ref10","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-010-1347-6"},{"key":"S0031182014001115_ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2010.04.054"},{"key":"S0031182014001115_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.06.049"},{"key":"S0031182014001115_ref16","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2000.170"},{"key":"S0031182014001115_ref21","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6.10.986"},{"key":"S0031182014001115_ref49","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2007.11.019"},{"key":"S0031182014001115_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2007.05.015"},{"key":"S0031182014001115_ref40","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.2002.1020"},{"key":"S0031182014001115_ref20","doi-asserted-by":"publisher","DOI":"10.1007\/s10238-013-0229-6"},{"key":"S0031182014001115_ref45","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1396"},{"key":"S0031182014001115_ref36","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1998.00964.x"},{"key":"S0031182014001115_ref11","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840190629"},{"key":"S0031182014001115_ref43","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2008090999"},{"key":"S0031182014001115_ref2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2009.01157.x"},{"key":"S0031182014001115_ref32","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90019-5"},{"key":"S0031182014001115_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(11)62035-0"},{"key":"S0031182014001115_ref28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2006.04658.x"},{"key":"S0031182014001115_ref41","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra012396"},{"key":"S0031182014001115_ref44","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.10.5178"},{"key":"S0031182014001115_ref38","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v18.i18.2280"},{"key":"S0031182014001115_ref46","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70521-7"},{"key":"S0031182014001115_ref42","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510270416"},{"key":"S0031182014001115_ref8","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/283726"},{"key":"S0031182014001115_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(85)90051-7"},{"key":"S0031182014001115_ref4","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700271"},{"key":"S0031182014001115_ref50","first-page":"14","article-title":"Prevention and treatment of HBV reinfection following liver transplantation","volume":"10","author":"Yuan","year":"2002","journal-title":"Zhonghua Gan Zang Bing Za Zhi"},{"key":"S0031182014001115_ref33","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117617"},{"key":"S0031182014001115_ref19","doi-asserted-by":"publisher","DOI":"10.1089\/vim.2009.0067"},{"key":"S0031182014001115_ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2010.05.020"},{"key":"S0031182014001115_ref39","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2006.04149.x"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182014001115","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,21]],"date-time":"2019-04-21T04:15:01Z","timestamp":1555820101000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182014001115\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,11]]},"references-count":50,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["S0031182014001115"],"URL":"https:\/\/doi.org\/10.1017\/s0031182014001115","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,11]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2014\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}