{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:52:46Z","timestamp":1760122366854},"reference-count":21,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2014,8,11]],"date-time":"2014-08-11T00:00:00Z","timestamp":1407715200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Four valid species of <jats:italic>Sarcocystis<\/jats:italic> have been reported from the water buffalo (<jats:italic>Bubalus bubalis<\/jats:italic>): <jats:italic>Sarcocystis fusiformis<\/jats:italic>, <jats:italic>Sarcocystis buffalonis<\/jats:italic>, <jats:italic>Sarcocystis levinei<\/jats:italic> and <jats:italic>Sarcocystis dubeyi<\/jats:italic>. Here, we redescribe structure of <jats:italic>S. fusiformis<\/jats:italic> sarcocysts by scanning and transmission electron microscopy (SEM, TEM). Twenty-one macroscopic sarcocysts from oesophagus of the water buffalo in Egypt were examined by light microscopy, SEM and TEM. The sarcocyst wall was up to 9\u00a0<jats:italic>\u03bc<\/jats:italic>m thick, depending on the section and the technique. In 5\u00a0<jats:italic>\u03bc<\/jats:italic>m paraffin-embedded sections, the sarcocyst wall was indistinct, 2\u20135\u00a0<jats:italic>\u03bc<\/jats:italic>m thick and appeared smooth. In 1\u00a0<jats:italic>\u03bc<\/jats:italic>m plastic-embedded sections stained with toluidine blue, the sarcocyst wall was 2\u00b75\u20135\u00b72\u00a0<jats:italic>\u03bc<\/jats:italic>m thick and had branched villar protrusions (vp)-like branches of a dead tree. By SEM, the sarcocyst wall had a mesh-like structure with irregularly shaped vp that were folded over the sarcocyst wall. On each vp there were uniform papillomatous structures that were 100\u00a0nm wide. By TEM, vp were up to 6\u00a0<jats:italic>\u03bc<\/jats:italic>m long and contained filamentous tubular structures, most of which were parallel to the long axis of the projections; granules were absent from these tubules. By TEM, bradyzoites within the same cyst varied from 11\u00b72 to 16\u00b78\u00a0<jats:italic>\u03bc<\/jats:italic>m in length. By TEM, bradyzoites had a very long (10\u00a0<jats:italic>\u03bc<\/jats:italic>m) convoluted mitochondrion, up to 12 dense granules, but only 2 rhoptries. This redescription should help to differentiate the sarcocysts of <jats:italic>S. fusiformis<\/jats:italic> from similar sarcocysts in domestic and wild ruminants.<\/jats:p>","DOI":"10.1017\/s003118201400122x","type":"journal-article","created":{"date-parts":[[2014,8,11]],"date-time":"2014-08-11T09:23:40Z","timestamp":1407749020000},"page":"385-394","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":15,"title":["Redescription of <i>Sarcocystis fusiformis<\/i> sarcocysts from the water buffalo (<i>Bubalus bubalis<\/i>)"],"prefix":"10.1017","volume":"142","author":[{"given":"J. P.","family":"DUBEY","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"HILALI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"VAN WILPE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. K.","family":"VERMA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"CALERO-BERNAL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"ABDEL-WAHAB","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2014,8,11]]},"reference":[{"key":"S003118201400122X_ref13","doi-asserted-by":"publisher","DOI":"10.2307\/3284375"},{"key":"S003118201400122X_ref9","unstructured":"Huong L. T. T. (1999 a). Sarcocystis infections of the water buffalo in Vietnam. Doctoral thesis. Swedish University of Agricultural Sciences, Uppsala, Sweden, pp. 1\u201353."},{"key":"S003118201400122X_ref8","doi-asserted-by":"publisher","DOI":"10.1007\/BF00389639"},{"key":"S003118201400122X_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.02.004"},{"key":"S003118201400122X_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2014.06.020"},{"key":"S003118201400122X_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(89)90176-3"},{"key":"S003118201400122X_ref2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00384828"},{"key":"S003118201400122X_ref20","first-page":"489","article-title":"Fine structure of Sarcocystis fusiformis from the Indian water buffalo (Bubalus bubalis) in Singapore","volume":"3","author":"Zaman","year":"1972","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S003118201400122X_ref12","doi-asserted-by":"publisher","DOI":"10.2307\/3284413"},{"key":"S003118201400122X_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.12.026"},{"key":"S003118201400122X_ref18","first-page":"40","article-title":"Ultrastructural studies of the cyst wall of Sarcocystis spp. In Philippine carabaos","volume":"14","author":"Parairo","year":"1988","journal-title":"Philippine Journal of Veterinary and Animal Science"},{"key":"S003118201400122X_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.08.024"},{"key":"S003118201400122X_ref16","doi-asserted-by":"publisher","DOI":"10.2307\/3280101"},{"key":"S003118201400122X_ref15","doi-asserted-by":"publisher","DOI":"10.1007\/BF00927151"},{"key":"S003118201400122X_ref19","first-page":"356","article-title":"A comparative ultrastructural study on the two types of sarcocysts in Chinese buffaloes","volume":"20","author":"Wang","year":"1989","journal-title":"Acta Veterinaria et Zootechnica Sinica"},{"key":"S003118201400122X_ref6","first-page":"289","article-title":"Ultrastructural study of Sarcocystis fusiformis (Railliet,1897) infecting the Indian water buffalo (Bubalus bubalis) of Egypt","volume":"29","author":"Ghaffar","year":"1978","journal-title":"Tropenmedizin und Parasitologie"},{"key":"S003118201400122X_ref3","first-page":"1","volume-title":"Sarcocystosis of Animals and Man","author":"Dubey","year":"1989"},{"key":"S003118201400122X_ref21","first-page":"205","article-title":"Scanning electron microscopy of Sarcocystis fusiformis from the water buffalo (Bubalus bubalis)","volume":"11","author":"Zaman","year":"1980","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S003118201400122X_ref11","doi-asserted-by":"publisher","DOI":"10.2307\/3285709"},{"key":"S003118201400122X_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(99)00135-1"},{"key":"S003118201400122X_ref17","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-014-3877-x"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S003118201400122X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,21]],"date-time":"2019-04-21T00:15:42Z","timestamp":1555805742000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S003118201400122X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,11]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["S003118201400122X"],"URL":"https:\/\/doi.org\/10.1017\/s003118201400122x","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,11]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2014\u00a0This is a work of the U.S. Government and is not subject to copyright protection in the United States.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}