{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T07:55:26Z","timestamp":1793087726354,"version":"4.5.0"},"reference-count":20,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2015,11,2]],"date-time":"2015-11-02T00:00:00Z","timestamp":1446422400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Fasciola hepatica<\/jats:italic> is a parasitic trematode that causes serious losses to livestock producers, and also zoonotic disease. The limitations of chemotherapy for the control of fasciolosis have led to significant interest in the development of vaccines to protect cattle and sheep from infection. However, relatively few studies have concentrated on the mechanisms of invasion of the gut by newly excysted juvenile liver flukes (NEJ) and the host response triggered by this event. The aim of this work was to develop an <jats:italic>in vitro<\/jats:italic> model to study invasion by NEJ, while also reducing the requirement for challenge infections of experimental animals. <jats:italic>Fasciola hepatica<\/jats:italic> metacercariae were excysted <jats:italic>in vitro<\/jats:italic> and placed into compartments containing rat distal jejunal sheets. Variations in incubation medium, chamber size and incubation temperature were used to identify optimal conditions for NEJ migration across the gut. Histological examination showed increased migration until 120 min post-incubation. The use of RPMI, without gassing at 39 \u00b0C, as the incubation medium was found to be optimal, with 40\u00b75% of NEJ migrating after 150 min. This study describes a readily-reproducible method for studying the migration of <jats:italic>F. hepatica<\/jats:italic> NEJ within the definitive host. It will be useful for identifying potential drug and vaccine targets.<\/jats:p>","DOI":"10.1017\/s0031182015001481","type":"journal-article","created":{"date-parts":[[2015,11,2]],"date-time":"2015-11-02T09:10:03Z","timestamp":1446455403000},"page":"24-33","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":17,"title":["Development of a versatile <i>in vitro<\/i> method for understanding the migration of <i>Fasciola hepatica<\/i> newly excysted juveniles"],"prefix":"10.1017","volume":"143","author":[{"given":"ANDRES","family":"GARCIA-CAMPOS","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ALAN W.","family":"BAIRD","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GRACE","family":"MULCAHY","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2015,11,2]]},"reference":[{"key":"S0031182015001481_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(81)90036-9"},{"key":"S0031182015001481_ref9","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.54.69"},{"key":"S0031182015001481_ref18","doi-asserted-by":"publisher","DOI":"10.1258\/002367787781268693"},{"key":"S0031182015001481_ref15","doi-asserted-by":"publisher","DOI":"10.2307\/3284586"},{"key":"S0031182015001481_ref17","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.1999.00226.x"},{"key":"S0031182015001481_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(75)90036-3"},{"key":"S0031182015001481_ref2","doi-asserted-by":"publisher","DOI":"10.1080\/10611860410001693715"},{"key":"S0031182015001481_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(93)90180-N"},{"key":"S0031182015001481_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2008.04.020"},{"key":"S0031182015001481_ref5","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-11-227"},{"key":"S0031182015001481_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(93)90172-T"},{"key":"S0031182015001481_ref8","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/S0034-5288(18)33562-8","article-title":"Methods for in vitro study of the invasive processes of Fasciola gigantica","volume":"19","author":"Hanna","year":"1975","journal-title":"Research in Veterinary Science"},{"key":"S0031182015001481_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2007.10.007"},{"key":"S0031182015001481_ref11","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M900045-MCP200"},{"key":"S0031182015001481_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2006.06.021"},{"key":"S0031182015001481_ref7","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/S0034-5288(18)32558-X","article-title":"Hypersensitivity in rats infected with Fasciola hepatica: possible role in protection against a challenge infection","volume":"30","author":"Doy","year":"1981","journal-title":"Research in Veterinary Science"},{"key":"S0031182015001481_ref13","doi-asserted-by":"publisher","DOI":"10.2307\/3281496"},{"key":"S0031182015001481_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(98)00026-X"},{"key":"S0031182015001481_ref19","volume-title":"University Physics","author":"Young","year":"1992"},{"key":"S0031182015001481_ref20","doi-asserted-by":"publisher","DOI":"10.1007\/BF02121277"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182015001481","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,20]],"date-time":"2019-04-20T00:00:45Z","timestamp":1555718445000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182015001481\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,2]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["S0031182015001481"],"URL":"https:\/\/doi.org\/10.1017\/s0031182015001481","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,2]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2015\u00a0This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}