{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T15:34:08Z","timestamp":1790004848779,"version":"4.0.1"},"reference-count":39,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Ocular toxoplasmosis is the most frequent cause of uveitis, leading to partial or total loss of vision, with the retina the main affected structure. The cells of the retinal pigment epithelium (RPE) play an important role in the physiology of the retina and formation of the blood\u2013retinal barrier. Several pathogens induce barrier dysfunction by altering tight junction (TJ) integrity. Here, we analysed the effect of infection by<jats:italic>Toxoplasma gondii<\/jats:italic>on TJ integrity in ARPE-19 cells. Loss of TJ integrity was demonstrated in<jats:italic>T. gondii<\/jats:italic>-infected ARPE-19 cells, causing increase in paracellular permeability and disturbance of the barrier function of the RPE. Confocal microscopy also revealed alteration in the TJ protein occludin induced by<jats:italic>T. gondii<\/jats:italic>infection. Disruption of junctional complex was also evidenced by scanning and transmission electron microscopy. Cell\u2013cell contact loss was noticed in the early stages of infection by<jats:italic>T. gondii<\/jats:italic>with the visualization of small to moderate intercellular spaces. Large gaps were mostly observed with the progression of the infection. Thus, our data suggest that the alterations induced by<jats:italic>T. gondii<\/jats:italic>in the structural organization of the RPE may contribute to retinal injury evidenced by ocular toxoplasmosis.<\/jats:p>","DOI":"10.1017\/s0031182015001973","type":"journal-article","created":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T06:19:38Z","timestamp":1456813178000},"page":"568-575","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":26,"title":["Effect of<i>Toxoplasma gondii<\/i>infection on the junctional complex of retinal pigment epithelial cells"],"prefix":"10.1017","volume":"143","author":[{"given":"ALANDERSON 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