{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:49:45Z","timestamp":1791982185023,"version":"4.4.0"},"reference-count":66,"publisher":"Cambridge University Press (CUP)","issue":"8","license":[{"start":{"date-parts":[[2016,4,5]],"date-time":"2016-04-05T00:00:00Z","timestamp":1459814400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>A multi-marker nuclear genotyping approach was performed on larval and adult specimens of<jats:italic>Anisakis<\/jats:italic>spp. (<jats:italic>N<\/jats:italic>= 689) collected from fish and cetaceans in allopatric and sympatric areas of the two species<jats:italic>Anisakis pegreffii<\/jats:italic>and<jats:italic>Anisakis simplex<\/jats:italic>(s. s.), in order to: (1) identify specimens belonging to the parental taxa by using nuclear markers (allozymes loci) and sequence analysis of a new diagnostic nuclear DNA locus (i.e. partial sequence of the EF1<jats:italic>\u03b1<\/jats:italic>\u22121<jats:italic>n<\/jats:italic>DNA region) and (2) recognize hybrid categories. According to the Bayesian clustering algorithms, based on those markers, most of the individuals (<jats:italic>N<\/jats:italic>= 678) were identified as the parental species [i.e.<jats:italic>A. pegreffii<\/jats:italic>or<jats:italic>A. simplex<\/jats:italic>(s. s.)], whereas a smaller portion (<jats:italic>N<\/jats:italic>= 11) were recognized as F<jats:sub>1<\/jats:sub>hybrids. Discordant results were obtained when using the polymerase chain reaction\u2013restriction fragment length polymorphisms (PCR\u2013RFLPs) of the internal transcribed spacer (ITS) ribosomal DNA (rDNA) on the same specimens, which indicated the occurrence of a large number of \u2018hybrids\u2019 both in sympatry and allopatry. These findings raise the question of possible misidentification of specimens belonging to the two parental<jats:italic>Anisakis<\/jats:italic>and their hybrid categories derived from the application of that single marker (i.e. PCR\u2013RFLPs analysis of the ITS of rDNA). Finally, Bayesian clustering, using allozymes and EF1<jats:italic>\u03b1<\/jats:italic>\u22121<jats:italic>n<\/jats:italic>DNA markers, has demonstrated that hybridization between<jats:italic>A. pegreffii<\/jats:italic>and<jats:italic>A. simplex<\/jats:italic>(s. s.) is a contemporary phenomenon in sympatric areas, while no introgressive hybridization takes place between the two species.<\/jats:p>","DOI":"10.1017\/s0031182016000330","type":"journal-article","created":{"date-parts":[[2016,4,5]],"date-time":"2016-04-05T10:25:42Z","timestamp":1459851942000},"page":"998-1011","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":77,"title":["No more time to stay \u2018single\u2019 in the detection of<i>Anisakis pegreffii, A. simplex<\/i>(s. s.) and hybridization events between them: a multi-marker nuclear genotyping approach"],"prefix":"10.1017","volume":"143","author":[{"given":"S.","family":"MATTIUCCI","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"ACERRA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0950-0576","authenticated-orcid":false,"given":"M.","family":"PAOLETTI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"CIPRIANI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"LEVSEN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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