{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:22:37Z","timestamp":1758126157443},"reference-count":20,"publisher":"Cambridge University Press (CUP)","issue":"11","license":[{"start":{"date-parts":[[2016,5,25]],"date-time":"2016-05-25T00:00:00Z","timestamp":1464134400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>In the present study, a recombinant aminopeptidase P (rTgAPP) from <jats:italic>Toxoplasma gondii<\/jats:italic> was expressed in <jats:italic>Escherichia coli<\/jats:italic> to evaluate its enzyme parameters. The rTgAPP showed strong activity against a synthetic substrate for aminopeptidase P at pH 8\u00b70 with a <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> value of 0\u00b7255 <jats:italic>\u00b5<\/jats:italic><jats:sc>m<\/jats:sc> and a <jats:italic>k<\/jats:italic><jats:sub>cat<\/jats:sub> value of 35\u00b76 s<jats:sup>\u22121<\/jats:sup>. The overall catalytic efficiency (<jats:italic>k<\/jats:italic><jats:sub>cat<\/jats:sub>\/<jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub>) of the rTgAPP was 139\u00b76 \u00d7 10<jats:sup>5<\/jats:sup> M<jats:sup>\u22121<\/jats:sup> s<jats:sup>\u22121<\/jats:sup>. The activity of rTgAPP was enhanced by the addition of divalent cations and inhibited by bestatin. Deletion of TgAPP gene in the parasite through a CRISPR\/Cas9 system resulted in inhibition of growth indicating the importance of TgAPP. Thus our findings reveal that TgAPP is an active enzyme in <jats:italic>T. gondii<\/jats:italic> and provide an insight into the function of TgAPP.<\/jats:p>","DOI":"10.1017\/s0031182016000986","type":"journal-article","created":{"date-parts":[[2016,5,25]],"date-time":"2016-05-25T08:32:26Z","timestamp":1464165146000},"page":"1443-1449","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":9,"title":["Functional characterization of X-prolyl aminopeptidase from <i>Toxoplasma gondii<\/i>"],"prefix":"10.1017","volume":"143","author":[{"given":"MINGFA","family":"YANG","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JUN","family":"ZHENG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HONGLIN","family":"JIA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MINGXIN","family":"SONG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2016,5,25]]},"reference":[{"key":"S0031182016000986_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2014.09.003"},{"key":"S0031182016000986_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.12.049"},{"key":"S0031182016000986_ref17","first-page":"743","article-title":"Toxoplasmosis. 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