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In the present study, a monoclonal antibody (MoAb) against recombinant<jats:italic>Fasciola gigantica<\/jats:italic>fatty acid binding protein (rFgFABP) has been produced. As well, a reliable sandwich enzyme-linked immunosorbent assay (sandwich ELISA) has been developed for the detection of circulating FABP in the sera of mice experimentally and cattle naturally infected with<jats:italic>F. gigantica<\/jats:italic>. MoAb 3A3 and biotinylated rabbit anti-recombinant FABP antibody were selected due to their high reactivities and specificities. The lower detection limit of sandwich ELISA was 5 pg mL<jats:sup>\u22121<\/jats:sup>, and no cross-reaction with other parasite antigens was observed. This assay could detect<jats:italic>F. gigantica<\/jats:italic>infection from day 1 post infection. In experimental mice, the sensitivity, specificity and accuracy of this assay were 93\u00b73, 100 and 98\u00b72%, while in natural cattle they were 96\u00b77, 100 and 99\u00b71%. Hence, this sandwich ELISA method showed high efficiencies and precisions for diagnosis of fasciolosis by<jats:italic>F. gigantica<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0031182016001104","type":"journal-article","created":{"date-parts":[[2016,6,17]],"date-time":"2016-06-17T06:18:51Z","timestamp":1466144331000},"page":"1369-1381","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":13,"title":["Immunodiagnostic monoclonal antibody-based sandwich ELISA of fasciolosis by detection of<i>Fasciola gigantica<\/i>circulating fatty acid binding protein"],"prefix":"10.1017","volume":"143","author":[{"given":"PANAT","family":"ANURACPREEDA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RUNGLAWAN","family":"CHAWENGKIRTTIKUL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PRASERT","family":"SOBHON","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2016,6,17]]},"reference":[{"key":"S0031182016001104_ref56","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.2460\/ajvr.1985.46.07.1513","article-title":"Diagnosis of ovine fascioliasis by a dot enzyme-linked immunosorbent assay: a rapid microdiagnostic technique","volume":"46","author":"Zimmerman","year":"1985","journal-title":"American Journal of Veterinary Research"},{"key":"S0031182016001104_ref54","first-page":"153","article-title":"Diagnosis of cattle fasciolosis by the detection of a circulating antigen using a monoclonal antibody","volume":"15","author":"Viyanant","year":"1997","journal-title":"Asian Pacific Journal of Allergy and Immunology"},{"key":"S0031182016001104_ref52","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"S0031182016001104_ref50","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(87)90131-2"},{"key":"S0031182016001104_ref46","first-page":"387","article-title":"Fasciola gigantica: studies of the tegument as a basis for the developments of immunodiagnosis and vaccine","volume":"29","author":"Sobhon","year":"1998","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0031182016001104_ref45","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1996.4098"},{"key":"S0031182016001104_ref44","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(02)00007-9"},{"key":"S0031182016001104_ref43","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(92)90202-L"},{"key":"S0031182016001104_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.07.010"},{"key":"S0031182016001104_ref30","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(77)90092-3"},{"key":"S0031182016001104_ref28","first-page":"547","article-title":"Flotation on the Sellotape (demonstration)","volume":"66","author":"Hammond","year":"1972","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"key":"S0031182016001104_ref25","doi-asserted-by":"publisher","DOI":"10.1023\/B:VERC.0000047497.57392.8c"},{"key":"S0031182016001104_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(94)00718-R"},{"key":"S0031182016001104_ref21","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.1990.9694239"},{"key":"S0031182016001104_ref18","unstructured":"Chicz R. 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