{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:44:36Z","timestamp":1789987476846,"version":"4.0.1"},"reference-count":47,"publisher":"Cambridge University Press (CUP)","issue":"12","license":[{"start":{"date-parts":[[2016,7,15]],"date-time":"2016-07-15T00:00:00Z","timestamp":1468540800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["journals.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>In this study, the prevalence of piroplasms in dogs was assessed using polymerase chain reaction (PCR) to identify<jats:italic>Babesia<\/jats:italic>and<jats:italic>Theileria<\/jats:italic>species in 200 dogs from Northern and Central Tunisia between spring and autumn 2014. The overall molecular prevalence for piroplasms was 14\u00b75% \u00b1 0\u00b705 (29\/200); PCR detected 2 species, namely<jats:italic>Babesia vogeli<\/jats:italic>and<jats:italic>Theileria annulata<\/jats:italic>with an overall prevalence of 12\u00b75 \u00b1 0\u00b704 and 2% \u00b1 0\u00b702, respectively. No differences in the molecular prevalences of<jats:italic>B. vogeli<\/jats:italic>were revealed for age and sex (<jats:italic>P<\/jats:italic>&gt; 0\u00b705). The molecular prevalence of<jats:italic>B. vogeli<\/jats:italic>was significantly higher in central Tunisia (26\u00b75% \u00b1 0\u00b701) compared with the North (9\u00b76% \u00b1 0\u00b704) (<jats:italic>P<\/jats:italic>&lt; 0\u00b705). More working and companion dogs were infected by<jats:italic>B. vogeli<\/jats:italic>(25\u00b78 \u00b1 0\u00b715 and 21\u00b71% \u00b1 0\u00b713, respectively) in comparison with guarding dogs (1\u00b78% \u00b1 0\u00b703) (<jats:italic>P<\/jats:italic>&lt; 0\u00b705). Concerning<jats:italic>T. annulata,<\/jats:italic>no significant variation was observed for all studied risk factors (<jats:italic>P<\/jats:italic>&gt; 0\u00b705). Comparison of the partial sequences of<jats:italic>18S rRNA<\/jats:italic>and<jats:italic>Tams 1<\/jats:italic>genes confirmed the presence of 2 novel<jats:italic>B. vogeli<\/jats:italic>and<jats:italic>T. annulata<\/jats:italic>genotypes. This is the first molecular detection of<jats:italic>T. annulata<\/jats:italic>and genetic characterization of dogs\u2019 piroplasms in Tunisia. Further studies are needed to better assess the epidemiological feature of piroplasms infection in North Africa.<\/jats:p>","DOI":"10.1017\/s003118201600113x","type":"journal-article","created":{"date-parts":[[2016,7,15]],"date-time":"2016-07-15T10:16:45Z","timestamp":1468577805000},"page":"1622-1628","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":11,"title":["Molecular prevalence and genetic characterization of piroplasms in dogs from Tunisia"],"prefix":"10.1017","volume":"143","author":[{"given":"MOHAMED R.","family":"RJEIBI","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SAFA","family":"AMAIRIA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MARIEM","family":"ROUATBI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FATMA","family":"BEN SALEM","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MOEZ","family":"MABROUK","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MOHAMED","family":"GHARBI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2016,7,15]]},"reference":[{"key":"S003118201600113X_ref47","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.1989.9694194"},{"key":"S003118201600113X_ref48","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050445"},{"key":"S003118201600113X_ref45","unstructured":"The World Organisation for Animal Health\/WAHID Interface. 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