{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T08:14:39Z","timestamp":1779351279350,"version":"3.51.4"},"reference-count":39,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2016,11,29]],"date-time":"2016-11-29T00:00:00Z","timestamp":1480377600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Strongyloides venezuelensis<\/jats:italic>is a parasitic nematode of rodents that is frequently used to obtain heterologous antigens for immunological diagnosis of human strongyloidiasis. The aim of this study was to identify antigens from filariform larvae of<jats:italic>S. venezuelensis<\/jats:italic>for immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from filariform larvae of<jats:italic>S. venezuelensis<\/jats:italic>were obtained in phosphate saline (SS and SM) and in Tris\u2013HCl buffer (TS and TM), and were analysed by Western blotting. Different antigenic components were recognized by IgG antibodies from the sera of strongyloidiasis patients. Highest recognition was observed for a 30\u201340 kDa mass range present in all antigenic fractions. The band encompassing this mass range was then excised and subjected to mass spectrometry for protein identification. Immunoreactive proteins identified in the soluble fractions corresponded to metabolic enzymes, whereas cytoskeletal proteins and galectins were more abundant in the membrane fractions. These results represent the first approach towards identification of<jats:italic>S. venezuelensis<\/jats:italic>antigens for use in immunodiagnostic assays for human strongyloidiasis.<\/jats:p>","DOI":"10.1017\/s0031182016001645","type":"journal-article","created":{"date-parts":[[2016,11,29]],"date-time":"2016-11-29T08:00:39Z","timestamp":1480406439000},"page":"124-130","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":17,"title":["Potential immunological markers for diagnosis of human strongyloidiasis using heterologous antigens"],"prefix":"10.1017","volume":"144","author":[{"given":"M. A.","family":"CORRAL","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. M.","family":"PAULA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. M. C. 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