{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T13:53:48Z","timestamp":1762955628358},"reference-count":53,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2016,10,17]],"date-time":"2016-10-17T00:00:00Z","timestamp":1476662400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>There is an increasing interest in improving neurocysticercosis (NCC) diagnosis through the search of new and alternative antigenic sources, as those obtained from heterologous antigens. The aim of this study was to obtain potential biomarkers for NCC diagnosis after gel filtration chromatography [gel filtration fraction (GFF)] from the total saline extract (SE) from <jats:italic>Taenia saginata<\/jats:italic> metacestodes, followed by protein identification and application in immunodiagnostic. SE and GFF proteic profiles were characterized in gel electrophoresis, and diagnostic performance was verified by testing 160 serum samples through enzyme-linked immunosorbent assay and immunoblotting. Sensitivity (Se), specificity (Sp) and other diagnostic parameters were calculated. Polypeptides of interest in the diagnosis of human NCC present at GFF were analysed by mass spectrometry (MS) and B-cell epitopes were predicted. GFF had the best diagnostic parameters: Se 93\u00b73%; Sp 93%; AUC 0\u00b7990; LR+ = 13\u00b742 and LR\u2212 = 0\u00b707, and proved to be useful reacting with serum samples in immunoblotting. Proteic profile ranged from 64 to 68 kDa and enolase and calcium binding protein calreticulin precursor were identified after MS. The enolase and calcium-binding protein calreticulin precursor showed 18 and 10 predicted B-cell epitopes, respectively. In conclusion we identified important markers in the GFF with high efficiency to diagnose NCC.<\/jats:p>","DOI":"10.1017\/s0031182016001839","type":"journal-article","created":{"date-parts":[[2016,10,17]],"date-time":"2016-10-17T10:47:13Z","timestamp":1476701233000},"page":"426-435","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":9,"title":["Usefulness of gel filtration fraction as potential biomarker for neurocysticercosis in serum: towards a new diagnostic tool"],"prefix":"10.1017","volume":"144","author":[{"given":"D. S.","family":"NUNES","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. T.","family":"GONZAGA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. S.","family":"RIBEIRO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. P.","family":"CUNHA-J\u00daNIOR","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. M.","family":"COSTA-CRUZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2016,10,17]]},"reference":[{"key":"S0031182016001839_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(95)00121-P"},{"key":"S0031182016001839_ref15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022394"},{"key":"S0031182016001839_ref50","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-1913-z"},{"key":"S0031182016001839_ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2013.01.020"},{"key":"S0031182016001839_ref32","doi-asserted-by":"publisher","DOI":"10.1645\/GE-3325RN"},{"key":"S0031182016001839_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2007.09.014"},{"key":"S0031182016001839_ref41","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00516-09"},{"key":"S0031182016001839_ref35","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000870"},{"key":"S0031182016001839_ref20","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201000432"},{"key":"S0031182016001839_ref18","doi-asserted-by":"publisher","DOI":"10.1590\/S0037-86822010000300008"},{"key":"S0031182016001839_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2015.03.006"},{"key":"S0031182016001839_ref51","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(1950)3:1<32::AID-CNCR2820030106>3.0.CO;2-3"},{"key":"S0031182016001839_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"S0031182016001839_ref40","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-009-1379-z"},{"key":"S0031182016001839_ref39","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-007-0578-8"},{"key":"S0031182016001839_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2015.08.011"},{"key":"S0031182016001839_ref33","first-page":"507","article-title":"Contribui\u00e7\u00e3o para o estudo do Strongyloides stercoralis e da estrongiloid\u00edase no Brasil","volume":"1","author":"Moraes","year":"1948","journal-title":"Revista de Sa\u00fade P\u00fablica"},{"key":"S0031182016001839_ref22","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.143.1.7063747"},{"key":"S0031182016001839_ref8","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-014-9747-z"},{"key":"S0031182016001839_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2010.06.016"},{"key":"S0031182016001839_ref42","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2006.07.003"},{"key":"S0031182016001839_ref47","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1985.00360030074016"},{"key":"S0031182016001839_ref14","doi-asserted-by":"publisher","DOI":"10.1159\/000365847"},{"key":"S0031182016001839_ref26","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"S0031182016001839_ref31","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2015.10.011"},{"key":"S0031182016001839_ref34","first-page":"77","article-title":"Comparison of cysticercus extract, cyst fluid and Taenia saginata extract for use in ELISA for serodiagnosis of neurocysticercosis","volume":"23","author":"Morakote","year":"1992","journal-title":"Southeast Asian Ministers of Education Council Central Coordinating Board for Tropical Medicine and Public Health"},{"key":"S0031182016001839_ref28","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2007.01917.x"},{"key":"S0031182016001839_ref16","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.7.3178-3184.2005"},{"key":"S0031182016001839_ref44","first-page":"326","article-title":"An ether sedimentation technique for rotine stool examination","volume":"8","author":"Ritchie","year":"1948","journal-title":"Bulletin of the National Service of Army \u2013 Medical Department"},{"key":"S0031182016001839_ref49","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2013.03.008"},{"key":"S0031182016001839_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.02.075"},{"key":"S0031182016001839_ref12","doi-asserted-by":"publisher","DOI":"10.1179\/2047773212Y.0000000025"},{"key":"S0031182016001839_ref27","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761919000100006"},{"key":"S0031182016001839_ref5","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762007000600009"},{"key":"S0031182016001839_ref7","doi-asserted-by":"publisher","DOI":"10.1111\/epi.12978"},{"key":"S0031182016001839_ref38","first-page":"14","article-title":"Cysticercosis-related hospitalizations in the United States, 1998\u20132011","volume":"10","author":"O'keefe","year":"2014","journal-title":"The American Journal of Tropical Medicine and Hygiene"},{"key":"S0031182016001839_ref45","doi-asserted-by":"publisher","DOI":"10.1016\/j.apjtm.2015.12.020"},{"key":"S0031182016001839_ref43","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-014-3908-7"},{"key":"S0031182016001839_ref19","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12307"},{"key":"S0031182016001839_ref48","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"S0031182016001839_ref52","doi-asserted-by":"publisher","DOI":"10.1016\/S1672-0229(10)60004-6"},{"key":"S0031182016001839_ref53","doi-asserted-by":"crossref","first-page":"964","DOI":"10.4269\/ajtmh.2009.80.964","article-title":"Utility of a protein fraction with cathepsin L-Like activity purified from cysticercus fluid of Taenia solium in the diagnosis of human cysticercosis","volume":"80","author":"Zimic","year":"2009","journal-title":"The American Journal of Tropical Medicine and Hygiene"},{"key":"S0031182016001839_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2006.09.006"},{"key":"S0031182016001839_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.wneu.2012.11.025"},{"key":"S0031182016001839_ref11","first-page":"337","article-title":"Spinal fluid immunoenzymatic assay (ELISA) for neurocysticercosis","volume":"24","author":"Costa","year":"1982","journal-title":"Revista do Instituto de Medicina Tropical de S\u00e3o Paulo"},{"key":"S0031182016001839_ref23","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.148.3.6878708"},{"key":"S0031182016001839_ref3","unstructured":"Baermann G. (1917). Eine einfache Methode zur Auffindung von Ankylostomum (Nematoden) Larven in Erdproben Mededeel mit H Geneesk Laboratories Weltevreden, Feestbundel, Batavia, pp. 41\u201347."},{"key":"S0031182016001839_ref46","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.2000.00567.x"},{"key":"S0031182016001839_ref10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003919"},{"key":"S0031182016001839_ref4","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.2001.00701.x"},{"key":"S0031182016001839_ref25","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1994.03510330081039"},{"key":"S0031182016001839_ref30","first-page":"7","article-title":"A curva ROC para testes diagn\u00f3sticos","volume":"11","author":"Martinez","year":"2003","journal-title":"Cadernos de Sa\u00fade Coletiva"},{"key":"S0031182016001839_ref1","article-title":"New insights in cysticercosis transmission","volume":"10","author":"Arriola","year":"2014","journal-title":"PLoS Neglected Tropical Diseases"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182016001839","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,18]],"date-time":"2019-04-18T01:41:06Z","timestamp":1555551666000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182016001839\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,17]]},"references-count":53,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["S0031182016001839"],"URL":"https:\/\/doi.org\/10.1017\/s0031182016001839","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,17]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2016\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}