{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:10:05Z","timestamp":1790712605064,"version":"4.1.0"},"reference-count":21,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2016,11,21]],"date-time":"2016-11-21T00:00:00Z","timestamp":1479686400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The primary causative agent of eosinophilic meningoencephalitis (EoM) in endemic regions is the nematode <jats:italic>Angiostrongylus cantonensis.<\/jats:italic> The occurrence of EoM was previously restricted to countries in Southeast Asia and the Pacific Islands; however, more recently, it has been reported from other regions, including Brazil. The commonly used diagnosis is detection of specific antibody reactivity to the 31 kDa antigen, which is derived from female worm somatic extracts. Here we report the occurrence of cross-reactivity to this antigen in sera from other parasitic infections, especially those that may cause EoM, such as gnathostomiasis, toxocariasis, hydatidosis and strongyloidiasis. We also demonstrated that the cross-reactivity, in part, is dependent of the concentration of antigen used in Western blot assays. We discuss the importance of these findings on the interpretation of this test.<\/jats:p>","DOI":"10.1017\/s0031182016001918","type":"journal-article","created":{"date-parts":[[2016,11,21]],"date-time":"2016-11-21T03:54:13Z","timestamp":1479700453000},"page":"459-463","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":19,"title":["Cross-reactivity of the 31 kDa antigen of <i>Angiostrongylus cantonensis<\/i> \u2013 Dealing with the immunodiagnosis of meningoencephalitis"],"prefix":"10.1017","volume":"144","author":[{"given":"ALESSANDRA L.","family":"MORASSUTTI","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LISA N.","family":"RASCOE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SUKWAN","family":"HANDALI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ALEXANDRE J.","family":"DA SILVA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PATRICIA P.","family":"WILKINS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CARLOS","family":"GRAEFF-TEIXEIRA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2016,11,21]]},"reference":[{"key":"S0031182016001918_ref5","first-page":"9","article-title":"Characterization of 31 kda specific Antigen from Parastrongylus cantonensis (Nematoda: Metastrongylidae)","volume":"2","author":"Eamsobhana","year":"1998","journal-title":"Journal of International Medical Research"},{"key":"S0031182016001918_ref9","doi-asserted-by":"publisher","DOI":"10.1645\/0022-3395(2002)088[1024:PACNEI]2.0.CO;2"},{"key":"S0031182016001918_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2016.04.012"},{"key":"S0031182016001918_ref17","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2009.09-0309"},{"key":"S0031182016001918_ref15","first-page":"291","article-title":"The evaluation of the 29 and 31 kDa antigens in female A. cantonensis for serodiagnosis of human angiostrongyliasis","volume":"27","author":"Nuamtanong","year":"1996","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0031182016001918_ref12","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2011.0957"},{"key":"S0031182016001918_ref3a","doi-asserted-by":"publisher","DOI":"10.1016\/j.crad.2004.05.012"},{"key":"S0031182016001918_ref7","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652013000200012"},{"key":"S0031182016001918_ref6","first-page":"308","article-title":"Purification of a specific immunodiagnostic Parastrongylus cantonensis antigen by electroelution from SDS-polyacrylamide gels","volume":"32","author":"Eamsobhana","year":"2001","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0031182016001918_ref8","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00044-08"},{"key":"S0031182016001918_ref11","doi-asserted-by":"crossref","first-page":"425","DOI":"10.4269\/ajtmh.2004.70.425","article-title":"Fatal autochthonous eosinophilic meningitis in a Jamaican child caused by Angiostrongylus cantonensis","volume":"70","author":"Lindo","year":"2004","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"S0031182016001918_ref13","first-page":"58","article-title":"Expression of recombinant antigenic proteins from Angiostrongylus cantonensis: a brief report","volume":"72","author":"Morassutti","year":"2013","journal-title":"Hawai'i Journal of Medicine and Public Health"},{"key":"S0031182016001918_ref2a","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1097\/00029330-200801010-00013","article-title":"Magnetic resonance imaging of eosinophilic meningoencephalitis caused by Angiostrongylus cantonensis following eating freshwater snails","volume":"121","author":"Jin","year":"2008","journal-title":"Chinese Medical Jounal"},{"key":"S0031182016001918_ref16","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.15-0146"},{"key":"S0031182016001918_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70229-9"},{"key":"S0031182016001918_ref14","doi-asserted-by":"publisher","DOI":"10.1590\/0074-0276140023"},{"key":"S0031182016001918_ref1","first-page":"173","article-title":"Angiostrongylus cantonensis. 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