{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T12:02:37Z","timestamp":1769169757553,"version":"3.49.0"},"reference-count":69,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2016,11,23]],"date-time":"2016-11-23T00:00:00Z","timestamp":1479859200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Entamoeba histolytica<\/jats:italic>is the causative agent of amoebic liver abscess (ALA), which course with an uncontrolled inflammation and nitro-oxidative stresses, although it is well known that amoeba has an effective defence mechanisms against this toxic environment, the underlying molecular factors responsible for progression of tissue damage remain largely unknown. The purpose of the present study was to determine during the acute stage of ALA in hamsters, the involvement of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and nuclear factor-kappa B (NF-\u03baB), which are activated in response to oxidative stress. From 12 h post-infection the ALA was visible, haematoxylin-eosin and Masson's trichrome stains were consistent with these observations, and alanine aminotransferase, alkaline phosphatase and \u03b3-glutamyl transpeptidase serum activities were increased too. At 48 h after infection, liver glycogen content was significantly reduced. Western blot analyses showed that 4-Hydroxy-2-nonenal peaked at 12 h, while glycogen synthase kinase-3\u03b2, cleaved caspase-3, pNF-\u03baB, interleukin-1\u03b2 and tumour necrosis factor-\u03b1 were overexpressed from 12 to 48 h post-infection. Otherwise, Nrf2 and superoxide dismutase-1, decreased at 48 h and catalase declined at 36 and 48 h. Furthermore, heme oxygenase-1 was increased at 12 and 24 h and decreased to normal levels at 36 and 48 h. These findings suggest for the first time that the host antioxidant system of Nrf2 is influenced during ALA.<\/jats:p>","DOI":"10.1017\/s0031182016001967","type":"journal-article","created":{"date-parts":[[2016,11,23]],"date-time":"2016-11-23T09:42:26Z","timestamp":1479894146000},"page":"384-393","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":4,"title":["Antioxidant defense of Nrf2<i>vs<\/i>pro-inflammatory system of NF-\u03baB during the amoebic liver infection in hamster"],"prefix":"10.1017","volume":"144","author":[{"given":"LISETH R.","family":"ALDABA-MURUATO","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MARTIN H.","family":"MU\u00d1OZ-ORTEGA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MAR\u00cdA DEL R.","family":"CAMPOS-ESPARZA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JOS\u00c9 R.","family":"MAC\u00cdAS-P\u00c9REZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"NAYELI A.","family":"M\u00c1RQUEZ-MU\u00d1OZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ANA G.","family":"VILLALOBOS-SANTOS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JAVIER","family":"VENTURA-JU\u00c1REZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2016,11,23]]},"reference":[{"key":"S0031182016001967_ref18","doi-asserted-by":"publisher","DOI":"10.1007\/s12664-012-0192-2"},{"key":"S0031182016001967_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(91)90192-6"},{"key":"S0031182016001967_ref52","first-page":"191","article-title":"The estimation of glycogen with the anthrone reagent","volume":"25","author":"Seifter","year":"1950","journal-title":"Arch Biochem"},{"key":"S0031182016001967_ref60","first-page":"112","article-title":"Inflammatory reaction in experimental hepatic amebiasis. An ultrastructural study","volume":"130","author":"Tsutsumi","year":"1988","journal-title":"American Journal of Pathology"},{"key":"S0031182016001967_ref47","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01204-10"},{"key":"S0031182016001967_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"S0031182016001967_ref33","volume-title":"Manual of Histologic Staining Methods of the Arme Forces Institute of Pathology","author":"Luna","year":"1968"},{"key":"S0031182016001967_ref12","doi-asserted-by":"publisher","DOI":"10.1517\/13543780802716501"},{"key":"S0031182016001967_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2004.09.003"},{"key":"S0031182016001967_ref19","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05344.x"},{"key":"S0031182016001967_ref11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2003.07486.x"},{"key":"S0031182016001967_ref7","first-page":"651","article-title":"Catalases \u2013 with and without heme","volume":"49","author":"Beyer","year":"1988","journal-title":"Basic Life Sciences"},{"key":"S0031182016001967_ref2","doi-asserted-by":"publisher","DOI":"10.3233\/NIB-150108"},{"key":"S0031182016001967_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)01308-1"},{"key":"S0031182016001967_ref31","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M611336200"},{"key":"S0031182016001967_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(78)90144-X"},{"key":"S0031182016001967_ref40","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002254"},{"key":"S0031182016001967_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(80)90209-X"},{"key":"S0031182016001967_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2013.04.001"},{"key":"S0031182016001967_ref43","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2010.01252.x"},{"key":"S0031182016001967_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2010.01.001"},{"key":"S0031182016001967_ref55","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-3231.2011.02604.x"},{"key":"S0031182016001967_ref41","doi-asserted-by":"publisher","DOI":"10.1139\/o11-084"},{"key":"S0031182016001967_ref30","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2000.00085.x"},{"key":"S0031182016001967_ref66","first-page":"13","article-title":"WHO\/PAHO\/UNESCO report. A consultation with experts on amoebiasis. Mexico City, Mexico 28-29 January, 1997","volume":"18","year":"1997","journal-title":"Epidemiological Bulletin"},{"key":"S0031182016001967_ref65","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.122062299"},{"key":"S0031182016001967_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(01)00011-9"},{"key":"S0031182016001967_ref18a","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)79605-X"},{"key":"S0031182016001967_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(72)80075-9"},{"key":"S0031182016001967_ref59","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2009.09.024"},{"key":"S0031182016001967_ref3","doi-asserted-by":"publisher","DOI":"10.3390\/cancers2020483"},{"key":"S0031182016001967_ref6","doi-asserted-by":"crossref","first-page":"3615","DOI":"10.1128\/IAI.65.9.3615-3621.1997","article-title":"Necrosis versus apoptosis as the mechanism of target cell death induced by Entamoeba histolytica","volume":"65","author":"Berninghausen","year":"1997","journal-title":"Infection and Immunity"},{"key":"S0031182016001967_ref10","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2016006"},{"key":"S0031182016001967_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2010.12.011"},{"key":"S0031182016001967_ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-003-3206-5"},{"key":"S0031182016001967_ref16","doi-asserted-by":"publisher","DOI":"10.1080\/15216540600686896"},{"key":"S0031182016001967_ref17","first-page":"96","article-title":"The role of oxygen-nitrogen stress in pathogenesis of amoebiasis","volume":"131","author":"Chikobava","year":"2006","journal-title":"Georgian Medical News"},{"key":"S0031182016001967_ref22","doi-asserted-by":"publisher","DOI":"10.1042\/bj20021516"},{"key":"S0031182016001967_ref23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.1986.tb03288.x"},{"key":"S0031182016001967_ref27","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00152-10"},{"key":"S0031182016001967_ref28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003096"},{"key":"S0031182016001967_ref29","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.066035"},{"key":"S0031182016001967_ref32a","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2014001"},{"key":"S0031182016001967_ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2008.07.017"},{"key":"S0031182016001967_ref34","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2009.05.018"},{"key":"S0031182016001967_ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2013.10.819"},{"key":"S0031182016001967_ref38","volume-title":"Guide for the Care and Use of Laboratory Animals","year":"2011"},{"key":"S0031182016001967_ref39","first-page":"885","article-title":"Interaction of heme with nitroxyl or nitric oxide amplifies heme oxygenase-1 induction: involvement of the transcription factor Nrf2","volume":"48","author":"Naughton","year":"2002","journal-title":"Cellular and Molecular Biology"},{"key":"S0031182016001967_ref42","doi-asserted-by":"publisher","DOI":"10.4254\/wjh.v5.i10.541"},{"key":"S0031182016001967_ref44","doi-asserted-by":"crossref","first-page":"15","DOI":"10.18632\/genesandcancer.4","article-title":"SOD1, an unexpected novel target for cancer therapy","volume":"5","author":"Papa","year":"2014","journal-title":"Genes Cancer"},{"key":"S0031182016001967_ref45","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(05)96028-X"},{"key":"S0031182016001967_ref46","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.423467"},{"key":"S0031182016001967_ref48","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-005-4503-z"},{"key":"S0031182016001967_ref49","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/28.1.56"},{"key":"S0031182016001967_ref50","doi-asserted-by":"publisher","DOI":"10.1159\/000362240"},{"key":"S0031182016001967_ref51","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00029-08"},{"key":"S0031182016001967_ref53","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/890603"},{"key":"S0031182016001967_ref54","doi-asserted-by":"crossref","first-page":"2980","DOI":"10.1128\/IAI.66.6.2980-2983.1998","article-title":"Entamoeba histolytica induces host cell death in amebic liver abscess by a non-Fas-dependent, non-tumor necrosis factor alpha-dependent pathway of apoptosis","volume":"66","author":"Seydel","year":"1998","journal-title":"Infection and Immunity"},{"key":"S0031182016001967_ref56","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206047200"},{"key":"S0031182016001967_ref57","first-page":"269","article-title":"NF-kappa B and Nrf2 as potential chemopreventive targets of some anti-inflammatory and antioxidative phytonutrients with anti-inflammatory and antioxidative activities","volume":"17","author":"Surh","year":"2008","journal-title":"Asia Pacific Journal of Clinical Nutrition"},{"key":"S0031182016001967_ref58","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.4.713-729.2003"},{"key":"S0031182016001967_ref62","doi-asserted-by":"publisher","DOI":"10.1139\/w01-136"},{"key":"S0031182016001967_ref63","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01236.x"},{"key":"S0031182016001967_ref64","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/8.2.228"},{"key":"S0031182016001967_ref67","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/460310"},{"key":"S0031182016001967_ref68","first-page":"27","article-title":"Oxidative stress promotes hepatocyte apoptosis mediated by glycogen synthase kinase 3\u03b2","volume":"31","author":"Zhang","year":"2015","journal-title":"Chinese Journal of Cellular and Molecular Immunology"},{"key":"S0031182016001967_ref35","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2012.04.011"},{"key":"S0031182016001967_ref61","first-page":"81","article-title":"Cellular bases of experimental amebic liver abscess formation","volume":"117","author":"Tsutsumi","year":"1984","journal-title":"American Journal of Pathology"},{"key":"S0031182016001967_ref5","first-page":"269","volume-title":"Methods of Enzymatic Analysis","author":"Bergmeyer","year":"1983"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182016001967","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,27]],"date-time":"2020-09-27T13:03:10Z","timestamp":1601211790000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182016001967\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,23]]},"references-count":69,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["S0031182016001967"],"URL":"https:\/\/doi.org\/10.1017\/s0031182016001967","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,23]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2016\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}