{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:42:29Z","timestamp":1791434549538,"version":"4.3.3"},"reference-count":20,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2016,11,29]],"date-time":"2016-11-29T00:00:00Z","timestamp":1480377600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Four species of malaria parasite, <jats:italic>Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae<\/jats:italic> and <jats:italic>Plasmodium knowlesi<\/jats:italic> infect humans living in the Khanh Phu commune, Khanh Hoa Province, Vietnam. The latter species also infects wild macaque monkeys in this region. In order to understand the transmission dynamics of the three species, we attempted to detect gametocytes of the three species in the blood of infected individuals, and sporozoites in the salivary glands of mosquitoes from the same region. For the detection of gametocyte-specific mRNA, we targeted region 3 of <jats:italic>pfg377, pvs25, pmg<\/jats:italic> and <jats:italic>pks25<\/jats:italic> as indicators of the presence of <jats:italic>P. falciparum, P. vivax, P. malariae<\/jats:italic> and <jats:italic>P. knowlesi<\/jats:italic> gametocytes, respectively. Gametocyte-specific mRNA was present in 37, 61, 0 and 47% of people infected with <jats:italic>P. falciparum<\/jats:italic> (<jats:italic>n<\/jats:italic> = 95), <jats:italic>P. vivax<\/jats:italic> (<jats:italic>n<\/jats:italic> = 69), <jats:italic>P. malariae<\/jats:italic> (<jats:italic>n<\/jats:italic> = 6) or <jats:italic>P. knowlesi<\/jats:italic> (<jats:italic>n<\/jats:italic> = 32), respectively. We found that 70% of mosquitoes that had <jats:italic>P. knowlesi<\/jats:italic> in their salivary glands also carried human malaria parasites, suggesting that mosquitoes are infected with <jats:italic>P. knowlesi<\/jats:italic> from human infections.<\/jats:p>","DOI":"10.1017\/s0031182016002110","type":"journal-article","created":{"date-parts":[[2016,11,29]],"date-time":"2016-11-29T03:00:25Z","timestamp":1480388425000},"page":"527-535","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":17,"title":["<i>Plasmodium knowlesi<\/i> and human malaria parasites in Khan Phu, Vietnam: Gametocyte production in humans and frequent co-infection of mosquitoes"],"prefix":"10.1017","volume":"144","author":[{"given":"Y.","family":"MAENO","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"CULLETON","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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