{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:47:21Z","timestamp":1777596441618,"version":"3.51.4"},"reference-count":29,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2016,12,12]],"date-time":"2016-12-12T00:00:00Z","timestamp":1481500800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Apical membrane antigen-1 (AMA-1) has been described as a potential vaccine candidate in apicomplexan parasites. Here we characterize the<jats:italic>ama-1<\/jats:italic>gene. The full-length<jats:italic>ama-1<\/jats:italic>gene of<jats:italic>Babesia<\/jats:italic>sp. BQ1 (Lintan) (BLTAMA-1) is 1785 bp, which contains an open reading frame (ORF) encoding a 65-kDa protein of 594 amino acid residues; by definition, the 5\u2032 UTR precedes the first methionine of the ORF. Phylogenetic analysis based on AMA-1 amino acid sequences clearly separated Piroplasmida from other Apicomplexa parasites. The<jats:italic>Babesia<\/jats:italic>sp. BQ1 (Lintan) AMA-1 sequence is most closely associated with that of<jats:italic>B. ovata<\/jats:italic>and<jats:italic>B. bigemina<\/jats:italic>, with high bootstrap value. A recombinant protein encoding a conserved region and containing ectodomains I and II of BLTAMA-1 was constructed. BLTrAMA-1-DI\/DII proteins were tested for reactivity with sera from sheep infected by<jats:italic>Babesia<\/jats:italic>sp. BQ1 (Lintan). In Western-blot analysis, native<jats:italic>Babesia<\/jats:italic>sp. BQ1 (Lintan) AMA-1 proteins were recognized by antibodies raised in rabbits against BLTrAMA-1<jats:italic>in vitro<\/jats:italic>. The results of this study are discussed in terms of gene characterization, taxonomy and antigenicity.<\/jats:p>","DOI":"10.1017\/s0031182016002304","type":"journal-article","created":{"date-parts":[[2016,12,12]],"date-time":"2016-12-12T02:24:47Z","timestamp":1481509487000},"page":"641-649","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":2,"title":["Molecular cloning, characterization and antigenicity of<i>Babesia<\/i>sp. BQ1 (Lintan) (<i>Babesia<\/i>cf.<i>motasi<\/i>) apical membrane antigen-1 (AMA-1)"],"prefix":"10.1017","volume":"144","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4782-5611","authenticated-orcid":false,"given":"QINGLI","family":"NIU","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ZHIJIE","family":"LIU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JIFEI","family":"YANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GUIQUAN","family":"GUAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YUPING","family":"PAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JIANXUN","family":"LUO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HONG","family":"YIN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2016,12,12]]},"reference":[{"key":"S0031182016002304_ref8","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182009991922"},{"key":"S0031182016002304_ref15","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0b013e328352245a"},{"key":"S0031182016002304_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2012.09.008"},{"key":"S0031182016002304_ref22","doi-asserted-by":"publisher","DOI":"10.1126\/science.1107449"},{"key":"S0031182016002304_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(92)90047-D"},{"key":"S0031182016002304_ref27","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-013-3310-x"},{"key":"S0031182016002304_ref14","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1128\/IAI.64.3.1054-1059.1996","article-title":"Identification 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