{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:26:10Z","timestamp":1762521970057,"version":"3.37.3"},"reference-count":22,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2017,1,11]],"date-time":"2017-01-11T00:00:00Z","timestamp":1484092800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Strongyloides stercoralis<\/jats:italic>is a parasite that can cause death in immunocompromised people. A proper diagnosis is hence essential. The real-time polymerase-chain reaction (RT\u2013PCR) is a novel, promising diagnostic method, that detects the DNA of the parasite in stool samples. In this retrospective study, we compared the sensitivity of agar plate coproculture (APC), an in-house immunofluorescence test (IFAT) and an in-house RT\u2013PCR for the diagnosis of<jats:italic>S. stercoralis<\/jats:italic>infection. The study sample was composed by 223 samples. Samples resulting positive to APC, IFAT and RT\u2013PCR were 20, 140 and 25, respectively. When sensitivity was calculated against a composite reference standard, serology confirmed the best performance (sensitivity 95%), followed by RT\u2013PCR (57%) and APC (45%). In conclusion, in a non-endemic setting, serology is the best screening method, while the combination of APC and RT\u2013PCR does not seem a reasonable approach to increase sensitivity. Both methods can have a role as confirmatory tests for selected cases.<\/jats:p>","DOI":"10.1017\/s0031182016002559","type":"journal-article","created":{"date-parts":[[2017,1,11]],"date-time":"2017-01-11T06:41:37Z","timestamp":1484116897000},"page":"812-816","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":46,"title":["A retrospective study comparing agar plate culture, indirect immunofluorescence and real-time PCR for the diagnosis of<i>Strongyloides stercoralis<\/i>infection"],"prefix":"10.1017","volume":"144","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0108-6822","authenticated-orcid":false,"given":"DORA","family":"BUONFRATE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"FRANCESCA","family":"PERANDIN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"FABIO","family":"FORMENTI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ZENO","family":"BISOFFI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2017,1,11]]},"reference":[{"key":"S0031182016002559_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2008.12.001"},{"key":"S0031182016002559_ref21","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.12-0437"},{"key":"S0031182016002559_ref20","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.15-0309"},{"key":"S0031182016002559_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2014.10.020"},{"key":"S0031182016002559_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/0732-8893(95)00247-2"},{"key":"S0031182016002559_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2013.02.003"},{"key":"S0031182016002559_ref14","doi-asserted-by":"publisher","DOI":"10.1086\/589718"},{"key":"S0031182016002559_ref12","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e3283551dbd"},{"key":"S0031182016002559_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(96)80806-4"},{"key":"S0031182016002559_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2011.08.010"},{"key":"S0031182016002559_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2015.04.001"},{"key":"S0031182016002559_ref4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002640"},{"key":"S0031182016002559_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2015.07.019"},{"key":"S0031182016002559_ref1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00041-14"},{"key":"S0031182016002559_ref7","doi-asserted-by":"publisher","DOI":"10.4321\/S1135-57272014000500004"},{"key":"S0031182016002559_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2016.02.004"},{"key":"S0031182016002559_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2012.12.012"},{"key":"S0031182016002559_ref8","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/11.5.755"},{"key":"S0031182016002559_ref13","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1128\/jcm.24.1.86-88.1986","article-title":"Elucidation of Strongyloides stercoralis by bacterial-colony displacement","volume":"24","author":"Panosian","year":"1986","journal-title":"Journal of Clinical Microbiology"},{"key":"S0031182016002559_ref11","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1991.45.518"},{"key":"S0031182016002559_ref3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002214"},{"key":"S0031182016002559_ref5","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00278-06"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182016002559","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T07:02:57Z","timestamp":1658386977000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182016002559\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,11]]},"references-count":22,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,5]]}},"alternative-id":["S0031182016002559"],"URL":"https:\/\/doi.org\/10.1017\/s0031182016002559","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"type":"print","value":"0031-1820"},{"type":"electronic","value":"1469-8161"}],"subject":[],"published":{"date-parts":[[2017,1,11]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2017\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}