{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T04:36:28Z","timestamp":1759638988487},"reference-count":33,"publisher":"Cambridge University Press (CUP)","issue":"7","license":[{"start":{"date-parts":[[2017,2,21]],"date-time":"2017-02-21T00:00:00Z","timestamp":1487635200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>In this study, we have produced a monoclonal antibody (MoAb) against 16 kDa antigen of<jats:italic>Paramphistomum gracile<\/jats:italic>(16 kDaAgPg), and developed an accurate sandwich enzyme-linked immunosorbent assay (sandwich ELISA) for the detection of circulating 16 kDaAg in the serum and fecal samples from cattle naturally infected with<jats:italic>P. gracile<\/jats:italic>. MoAb 1D10 was immobilized on a microtitre plate, and the antigen in the samples was captured and detected with biotinylated rabbit anti-16 kDaAgPg antibody. The lower detection limit of sandwich ELISA was 3\u00b75 pg mL<jats:sup>\u22121<\/jats:sup>, and no cross-reaction with other parasite antigens was evaluated. The reliability of the assay was examined using the serum and fecal samples from cattle naturally infected with<jats:italic>P. gracile, Fasciola gigantica, Moniezia benedeni, Trichuris<\/jats:italic>sp.,<jats:italic>Strongyloides<\/jats:italic>sp., strongylids and non-infected animals. The sandwich ELISA showed the sensitivity, specificity and accuracy at 98\u00b733, 100 and 99\u00b755% (serum samples), and 96\u00b767, 100 and 99\u00b709% (fecal samples). Therefore, this detection method is a rapid and excellent potential assay for the accurate diagnosis of paramphistomosis.<\/jats:p>","DOI":"10.1017\/s003118201600264x","type":"journal-article","created":{"date-parts":[[2017,2,21]],"date-time":"2017-02-21T14:25:50Z","timestamp":1487687150000},"page":"899-903","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":11,"title":["Immunodiagnosis of paramphistomosis using monoclonal antibody-based sandwich ELISA for detection of<i>Paramphistomum gracile<\/i>circulating 16 kDa antigen"],"prefix":"10.1017","volume":"144","author":[{"given":"PANAT","family":"ANURACPREEDA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KULLANID","family":"TEPSUPORNKUL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RUNGLAWAN","family":"CHAWENGKIRTTIKUL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2017,2,21]]},"reference":[{"key":"S003118201600264X_ref12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145650"},{"key":"S003118201600264X_ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-016-5273-1"},{"key":"S003118201600264X_ref23","first-page":"491","article-title":"A note on amphistomiasis in an adult buffalo","volume":"55","author":"Gupta","year":"1978","journal-title":"The Indian Veterinary Journal"},{"key":"S003118201600264X_ref28","doi-asserted-by":"publisher","DOI":"10.1645\/GE-192R"},{"key":"S003118201600264X_ref27","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"Journal of Chemical Biology"},{"key":"S003118201600264X_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60159-1"},{"key":"S003118201600264X_ref19","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-4-176"},{"key":"S003118201600264X_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.08.012"},{"key":"S003118201600264X_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.03.007"},{"key":"S003118201600264X_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-3955(16)33930-X"},{"key":"S003118201600264X_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.08.014"},{"key":"S003118201600264X_ref14","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-016-5024-3"},{"key":"S003118201600264X_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2014.12.003"},{"key":"S003118201600264X_ref13","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182016001104"},{"key":"S003118201600264X_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2013.03.020"},{"key":"S003118201600264X_ref34","doi-asserted-by":"crossref","first-page":"546","DOI":"10.4269\/ajtmh.1990.42.546","article-title":"Comparison of dot-ELISA with sandwich ELISA for the detection of circulating antigens in patients with Bancroftian filariasis","volume":"42","author":"Zheng","year":"1990","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"S003118201600264X_ref32","first-page":"27","article-title":"Prevalence of amphistomes of cattle in Thailand","volume":"29\/30","author":"Prasitirat","year":"1997","journal-title":"Parasitology (Hungary)"},{"key":"S003118201600264X_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2007.08.005"},{"key":"S003118201600264X_ref16","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182015001833"},{"key":"S003118201600264X_ref31","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.2013.14.4.425"},{"key":"S003118201600264X_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(88)90016-1"},{"key":"S003118201600264X_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2012.02.001"},{"key":"S003118201600264X_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2006.09.006"},{"key":"S003118201600264X_ref11","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12330"},{"key":"S003118201600264X_ref21","doi-asserted-by":"publisher","DOI":"10.1645\/GE-1672.1"},{"key":"S003118201600264X_ref18","unstructured":"Chethanon U. , Ausavamatha P. and Piriyayon S. 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