{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T07:51:46Z","timestamp":1778572306215,"version":"3.51.4"},"reference-count":20,"publisher":"Cambridge University Press (CUP)","issue":"7","license":[{"start":{"date-parts":[[2017,3,8]],"date-time":"2017-03-08T00:00:00Z","timestamp":1488931200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Alveolar echinococcosis (AE) is an emerging zoonotic disease caused by the cestode <jats:italic>Echinococcus multilocularis<\/jats:italic>. The secondary infection model of AE is based on intraperitoneal injection of disease-causing metacestodes into the peritoneal cavity of mice, which allows investigations on novel drugs or immunotherapeutical treatment options <jats:italic>in vivo<\/jats:italic>. So far, such <jats:italic>in vivo<\/jats:italic> studies assessed exclusively the parasite weight at the endpoint of a given treatment period. We here developed an ultrasound (US)-based scoring system that allows to follow-up parasite development in the living animal, and provides insights into parasite growth during the treatment phase. By this method a statistically significant difference between untreated and medicated mice with <jats:italic>E. multilocularis<\/jats:italic> infection was observed at 2 months post-infection, and the growth curve of the parasite load was described by a linear mixed model. High correlation and similar levels of variation were observed for the standard method based on parasite weight measurement, the novel US-based scoring system, as well volume segmentation by post-mortem magnetic resonance imaging. Thus, US-based scoring in the live animal has the potential to assist the 3R concept by contributing to the refinement and reduction of animal use in experimental echinococcosis.<\/jats:p>","DOI":"10.1017\/s0031182017000051","type":"journal-article","created":{"date-parts":[[2017,3,8]],"date-time":"2017-03-08T04:12:07Z","timestamp":1488946327000},"page":"937-944","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":10,"title":["To see or not to see: non-invasive imaging for improved readout of drug treatment trials in the murine model of secondary alveolar echinococcosis"],"prefix":"10.1017","volume":"144","author":[{"given":"D.","family":"GORGAS","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"MARREROS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"RUFENER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"HEMPHILL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2672-5766","authenticated-orcid":false,"given":"B.","family":"LUNDSTR\u00d6M-STADELMANN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2017,3,8]]},"reference":[{"key":"S0031182017000051_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2012.07.007"},{"key":"S0031182017000051_ref4","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-79054-1_7"},{"key":"S0031182017000051_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2015.07.027"},{"key":"S0031182017000051_ref2","first-page":"3","volume-title":"Boot: Bootstrap R (S-Plus) Functions. 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