{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:00:15Z","timestamp":1791388815750,"version":"4.3.3"},"reference-count":38,"publisher":"Cambridge University Press (CUP)","issue":"7","license":[{"start":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T00:00:00Z","timestamp":1489449600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>For the majority of intestinal parasites, real-time PCR-based diagnosis outperforms microscopy. However, the data for <jats:italic>Trichuris trichiura<\/jats:italic> have been less convincing and most comparative studies have been performed in populations with low prevalence. This study aims to improve detection of <jats:italic>T. trichuria<\/jats:italic> DNA in human stool by evaluating four sample preparation methods. Faecal samples (<jats:italic>n<\/jats:italic> = 60) were collected at Flores island, Indonesia and examined by microscopy. Aliquots were taken and a bead-beating procedure was used both on directly frozen stool and on material preserved with 96% ethanol. PCR on frozen samples showed 40% to be positive for <jats:italic>T. trichiura<\/jats:italic>, compared with 45% positive by microscopy. The percentage positive increased when using ethanol preservation (45\u00b70%), bead-beating (51\u00b77%) and a combination (55\u00b70%) and all three methods showed significantly higher DNA loads. The various procedures had a less pronounced effect on the PCR results of nine other parasite targets tested. Most prevalent were <jats:italic>Ascaris lumbricoides<\/jats:italic> (\u224860%), <jats:italic>Necator americanus<\/jats:italic> (\u224860%), <jats:italic>Dientamoeba fragilis<\/jats:italic> (\u224850%) and <jats:italic>Giardia lamblia<\/jats:italic> (\u224812%). To validate the practicality of the procedure, bead-beating was applied in a population-based survey testing 910 stool samples. Findings confirmed bead-beating before DNA extraction to be a highly efficient procedure for the detection of <jats:italic>T. trichiura<\/jats:italic> DNA in stool.<\/jats:p>","DOI":"10.1017\/s0031182017000129","type":"journal-article","created":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T09:04:57Z","timestamp":1489482297000},"page":"965-974","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":87,"title":["Improved diagnosis of <i>Trichuris trichiura<\/i> by using a bead-beating procedure on ethanol preserved stool samples prior to DNA isolation and the performance of multiplex real-time PCR for intestinal parasites"],"prefix":"10.1017","volume":"144","author":[{"given":"MARIA M. M.","family":"KAISAR","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ERIC A. 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\u00a9 Cambridge University Press 2017\u00a0This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}