{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T15:04:15Z","timestamp":1790089455992,"version":"4.0.1"},"reference-count":51,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2017,5,9]],"date-time":"2017-05-09T00:00:00Z","timestamp":1494288000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Little is known about the genetic diversity of the protozoan parasite,\n                    <jats:italic>Giardia duodenalis,<\/jats:italic>\n                    infecting humans in Queensland, Australia. The present study typed 88 microscopically\n                    <jats:italic>Giardia<\/jats:italic>\n                    -positive isolates using assemblage-specific primers at the triose phosphate isomerase (\n                    <jats:italic>tpi<\/jats:italic>\n                    ) gene and sequenced a subset of isolates at the glutamate dehydrogenase (\n                    <jats:italic>gdh<\/jats:italic>\n                    ) gene (\n                    <jats:italic>n<\/jats:italic>\n                    = 30) and\n                    <jats:italic>tpi<\/jats:italic>\n                    locus (\n                    <jats:italic>n<\/jats:italic>\n                    = 27). Using the\n                    <jats:italic>tpi<\/jats:italic>\n                    -assemblage specific primers,\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    assemblage A and assemblage B were detected in 50% (44\/88) and 38\u00b76% (34\/88) of samples, respectively. Mixed infections with assemblages A and B were identified in 4\u00b75% (4\/88) and assemblage E was identified in 6\u00b78% (6\/88) of samples. Sequence analysis at the\n                    <jats:italic>gdh<\/jats:italic>\n                    and\n                    <jats:italic>tpi<\/jats:italic>\n                    loci also confirmed the presence of assemblage E in these isolates. Cyst numbers per gram of feces (g\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) were determined using quantitative polymerase chain reaction and of the isolates that were typed as assemblage E, cyst numbers ranged 13\u00b78\u201368\u00b73 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    cysts g\n                    <jats:sup>\u22121<\/jats:sup>\n                    . This is the first report of assemblage E in humans in Australia, indicating that in certain settings, this assemblage may be zoonotic.\n                  <\/jats:p>","DOI":"10.1017\/s0031182017000439","type":"journal-article","created":{"date-parts":[[2017,5,9]],"date-time":"2017-05-09T05:02:56Z","timestamp":1494306176000},"page":"1154-1161","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":126,"title":["Molecular typing of\n                    <i>Giardia duodenalis<\/i>\n                    in humans in Queensland \u2013 first report of Assemblage 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