{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T21:57:05Z","timestamp":1790114225254,"version":"4.0.1"},"reference-count":24,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2017,5,8]],"date-time":"2017-05-08T00:00:00Z","timestamp":1494201600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Babesia<\/jats:italic> are intraerythrocytic parasites of importance worldwide within the fields of human and veterinary medicine, as some <jats:italic>Babesia<\/jats:italic> sp., including <jats:italic>Babesia microti<\/jats:italic> are potentially zoonotic and can cause fatal disease in both humans and animals. The aims of this study were to use a nested PCR (amplifying the 18S rRNA gene) to determine the presence and species of <jats:italic>Babesia<\/jats:italic> parasite DNA found in blood (<jats:italic>n<\/jats:italic> = 47) and spleen (<jats:italic>n<\/jats:italic> = 47) samples collected from Eurasian badgers (<jats:italic>Meles meles<\/jats:italic>) in Scotland. The results showed 28\/47 (59\u00b76%) blood and 14\/47 (29\u00b78%) spleen samples tested positive for the presence of <jats:italic>Babesia<\/jats:italic> DNA. Initial sequence analysis of the <jats:italic>Babesia<\/jats:italic> DNA identified three distinct sequence types (submitted to GenBank KX528553, KX528554 and KX528555), which demonstrated \u2a7e99% identity to <jats:italic>Babesia<\/jats:italic> sp. parasites previously identified in badgers in Spain (KT223484 and KT223485). Phylogenetic analysis showed that the three isolates are closely related to <jats:italic>Babesia annae, B. microti<\/jats:italic> and other Piroplasmida species found in wildlife. Further sequence analysis of the samples demonstrated that the badgers were routinely infected with more than one parasite isolate and there was also evidence of genetic recombination between the <jats:italic>Babesia<\/jats:italic> parasite isolates (submitted to GenBank KY250472 \u2013 KY250477)<\/jats:p>","DOI":"10.1017\/s0031182017000476","type":"journal-article","created":{"date-parts":[[2017,5,8]],"date-time":"2017-05-08T08:12:19Z","timestamp":1494231139000},"page":"1203-1210","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":15,"title":["Detection of <i>Babesia<\/i> DNA in blood and spleen samples from Eurasian badgers (<i>Meles meles<\/i>) in Scotland"],"prefix":"10.1017","volume":"144","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3351-7319","authenticated-orcid":false,"given":"PAUL M.","family":"BARTLEY","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CARI","family":"WILSON","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ELISABETH A.","family":"INNES","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FRANK","family":"KATZER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2017,5,8]]},"reference":[{"key":"S0031182017000476_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2014.12.035"},{"key":"S0031182017000476_ref9","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syq010"},{"key":"S0031182017000476_ref24","doi-asserted-by":"publisher","DOI":"10.1111\/1556-4029.12554"},{"key":"S0031182017000476_ref18","doi-asserted-by":"publisher","DOI":"10.1136\/vr.94.21.493"},{"key":"S0031182017000476_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2016.05.012"},{"key":"S0031182017000476_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(03)00078-5"},{"key":"S0031182017000476_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.02.021"},{"key":"S0031182017000476_ref1","doi-asserted-by":"publisher","DOI":"10.1080\/10635150600755453"},{"key":"S0031182017000476_ref5","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182013001169"},{"key":"S0031182017000476_ref7","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn180"},{"key":"S0031182017000476_ref4","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-016-1364-1"},{"key":"S0031182017000476_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2012.10.001"},{"key":"S0031182017000476_ref20","doi-asserted-by":"publisher","DOI":"10.1007\/s00442-014-3019-2"},{"key":"S0031182017000476_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2014.07.003"},{"key":"S0031182017000476_ref2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-0830-5"},{"key":"S0031182017000476_ref10","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000050149"},{"key":"S0031182017000476_ref11","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.3.451-469.2000"},{"key":"S0031182017000476_ref12","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2010.0079"},{"key":"S0031182017000476_ref13","doi-asserted-by":"publisher","DOI":"10.1038\/srep03809"},{"key":"S0031182017000476_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2015.10.029"},{"key":"S0031182017000476_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2015.11.003"},{"key":"S0031182017000476_ref19","unstructured":"Rainey E. , Butler A. , Bierman S. and Roberts A. M. I. (2009) Scottish Badger Distribution Survey 2006\u20132009: estimating the distribution and density of badger main setts in Scotland. Report prepared by Scottish Badgers and Biomathematics and Statistics Scotland."},{"key":"S0031182017000476_ref22","first-page":"372","article-title":"Piroplasmosis in a badger from Turkey","volume":"153","author":"Simsek","year":"2003","journal-title":"Veterinary Record"},{"key":"S0031182017000476_ref21","doi-asserted-by":"publisher","DOI":"10.1136\/vr.156.14.442"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182017000476","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T09:13:30Z","timestamp":1744794810000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182017000476\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,8]]},"references-count":24,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["S0031182017000476"],"URL":"https:\/\/doi.org\/10.1017\/s0031182017000476","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,8]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2017\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}