{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:15:46Z","timestamp":1790154946832,"version":"4.0.1"},"reference-count":22,"publisher":"Cambridge University Press (CUP)","issue":"11","license":[{"start":{"date-parts":[[2017,6,30]],"date-time":"2017-06-30T00:00:00Z","timestamp":1498780800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Toxoplasma gondii<\/jats:italic> is an obligate intracellular parasite of phylum Apicomplexa. To facilitate high-efficiency invasion of host cells, <jats:italic>T. gondii<\/jats:italic> secretes various proteins related to the moving junction (MJ) complex from rhoptries and micronemes into the interface between the parasite and host. AMA1\/RON2\/4\/5\/8 is an important MJ complex, but its mechanism of assembly remains unclear. In this study, we used the CRISPR-Cas9 system to generate a derivative of <jats:italic>T. gondii<\/jats:italic> strain RH with a null mutation in TgRON4, thought to be an essential MJ component. Deficiency of TgRON4 moderately decreased invasion ability relative to that of the wild-type parasite. In addition, expression of the endogenous <jats:italic>N<\/jats:italic>-terminal fragment of RON5 decreased in the mutant. Together, the results improve our understanding of the assembly mechanism of the MJ complex of <jats:italic>T. gondii<\/jats:italic> and raise the possibility of developing new therapeutic drugs that target this complex.<\/jats:p>","DOI":"10.1017\/s0031182017000968","type":"journal-article","created":{"date-parts":[[2017,6,30]],"date-time":"2017-06-30T08:15:29Z","timestamp":1498810529000},"page":"1490-1497","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":10,"title":["The moving junction protein RON4, although not critical, facilitates host cell invasion and stabilizes MJ members"],"prefix":"10.1017","volume":"144","author":[{"given":"MING","family":"WANG","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SHINUO","family":"CAO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"NALI","family":"DU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JIAWEN","family":"FU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ZHAORAN","family":"LI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HONGLIN","family":"JIA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MINGXIN","family":"SONG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2017,6,30]]},"reference":[{"key":"S0031182017000968_ref23","doi-asserted-by":"publisher","DOI":"10.1038\/srep34448"},{"key":"S0031182017000968_ref21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001282"},{"key":"S0031182017000968_ref20","doi-asserted-by":"publisher","DOI":"10.1038\/srep03199"},{"key":"S0031182017000968_ref17","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01114-14"},{"key":"S0031182017000968_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.09.099"},{"key":"S0031182017000968_ref15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0070637"},{"key":"S0031182017000968_ref14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1515898113"},{"key":"S0031182017000968_ref9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001276"},{"key":"S0031182017000968_ref5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000309"},{"key":"S0031182017000968_ref12","doi-asserted-by":"publisher","DOI":"10.1097\/NMD.0b013e318221416e"},{"key":"S0031182017000968_ref18","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3261"},{"key":"S0031182017000968_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.schres.2011.08.006"},{"key":"S0031182017000968_ref10","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5098"},{"key":"S0031182017000968_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.10.012"},{"key":"S0031182017000968_ref7","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1800"},{"key":"S0031182017000968_ref2","doi-asserted-by":"publisher","DOI":"10.1097\/NMD.0b013e3181c29a23"},{"key":"S0031182017000968_ref19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002007"},{"key":"S0031182017000968_ref6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2011.01597.x"},{"key":"S0031182017000968_ref11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00646.x"},{"key":"S0031182017000968_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2014.09.003"},{"key":"S0031182017000968_ref1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0010017"},{"key":"S0031182017000968_ref4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004025"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182017000968","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,17]],"date-time":"2019-04-17T00:40:39Z","timestamp":1555461639000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182017000968\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,30]]},"references-count":22,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["S0031182017000968"],"URL":"https:\/\/doi.org\/10.1017\/s0031182017000968","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,30]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2017\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}