{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T11:47:30Z","timestamp":1789645650574,"version":"4.0.0"},"reference-count":52,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2017,7,19]],"date-time":"2017-07-19T00:00:00Z","timestamp":1500422400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p><jats:italic>Plasmodium knowlesi<\/jats:italic>has risen in importance as a zoonotic parasite that has been causing regular episodes of malaria throughout South East Asia. The<jats:italic>P. knowlesi<\/jats:italic>genome sequence generated in 2008 highlighted and confirmed many similarities and differences in<jats:italic>Plasmodium<\/jats:italic>species, including a global view of several multigene families, such as the large<jats:italic>SICAvar<\/jats:italic>multigene family encoding the variant antigens known as the schizont-infected cell agglutination proteins. However, repetitive DNA sequences are the bane of any genome project, and this and other<jats:italic>Plasmodium<\/jats:italic>genome projects have not been immune to the gaps, rearrangements and other pitfalls created by these genomic features. Today, long-read PacBio and chromatin conformation technologies are overcoming such obstacles. Here, based on the use of these technologies, we present a highly refined<jats:italic>de novo P. knowlesi<\/jats:italic>genome sequence of the Pk1(A+) clone. This sequence and annotation, referred to as the \u2018MaHPIC Pk genome sequence\u2019, includes manual annotation of the<jats:italic>SICAvar<\/jats:italic>gene family with 136 full-length members categorized as type I or II. This sequence provides a framework that will permit a better understanding of the<jats:italic>SICAvar<\/jats:italic>repertoire, selective pressures acting on this gene family and mechanisms of antigenic variation in this species and other pathogens.<\/jats:p>","DOI":"10.1017\/s0031182017001329","type":"journal-article","created":{"date-parts":[[2017,7,19]],"date-time":"2017-07-19T08:22:47Z","timestamp":1500452567000},"page":"71-84","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":43,"title":["PacBio assembly of a<i>Plasmodium knowlesi<\/i>genome sequence with Hi-C correction and manual annotation of the<i>SICAvar<\/i>gene family"],"prefix":"10.1017","volume":"145","author":[{"given":"S. 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