{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T18:23:00Z","timestamp":1757701380956},"reference-count":36,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2017,9,25]],"date-time":"2017-09-25T00:00:00Z","timestamp":1506297600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>MicroRNAs (miRNAs) are short noncoding RNAs, involved in the regulation of parasite diseases. However, a role of miRNAs in<jats:italic>Echinococcus multilocularis<\/jats:italic>infection remains largely unknown. In this study, we first found the expression levels of key genes involved in miRNA biogenesis and function, including<jats:italic>Ago2<\/jats:italic>,<jats:italic>Xpo5<\/jats:italic>,<jats:italic>Tarbp2<\/jats:italic>and<jats:italic>DgcR8<\/jats:italic>, were obviously altered in the macrophage RAW264\u00b77 cells exposed to<jats:italic>E. multilocularis<\/jats:italic>metacestodes. Compared with the control, 18 and 32 known miRNAs were found to be differentially expressed (<jats:italic>P<\/jats:italic>&lt; 0\u00b705 and fold change &gt;2) in the macrophages exposed to<jats:italic>E. multilocularis<\/jats:italic>metacestodes for 6 and 12 h, respectively. Among these, several are known to be involved in regulating cytokine activities and immune responses. Quantitative real-time polymerase chain reaction results showed that the expression of nine selected miRNAs was consistent with the sequencing data at each treatment time points. Moreover, there were statistically significant correlations between the expression levels of miRNAs and their corresponding targeted genes. Our data give us some clues to pinpoint a role of miRNAs in the course of infection and immunity of<jats:italic>E. multilocularis.<\/jats:italic><\/jats:p>","DOI":"10.1017\/s0031182017001652","type":"journal-article","created":{"date-parts":[[2017,9,25]],"date-time":"2017-09-25T03:26:09Z","timestamp":1506309969000},"page":"416-423","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":7,"title":["MicroRNA expression profile in RAW264\u00b77 macrophage cells exposed to<i>Echinococcus multilocularis<\/i>metacestodes"],"prefix":"10.1017","volume":"145","author":[{"given":"XIAOLA","family":"GUO","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YADONG","family":"ZHENG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2017,9,25]]},"reference":[{"key":"S0031182017001652_ref17","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1477"},{"key":"S0031182017001652_ref18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0014557"},{"key":"S0031182017001652_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2009.07.001"},{"key":"S0031182017001652_ref21","first-page":"652","article-title":"Echinococcus multilocularis antigens modify accessory cell function of 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