{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T08:17:05Z","timestamp":1758269825860},"reference-count":30,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2017,11,27]],"date-time":"2017-11-27T00:00:00Z","timestamp":1511740800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Echinococcus granulosus<\/jats:italic> is an important zoonotic parasite that is distributed worldwide. The EG95 vaccine was developed to assist with control of <jats:italic>E<\/jats:italic>. <jats:italic>granulosus<\/jats:italic> transmission through the parasite's livestock intermediate hosts. The vaccine is based on a recombinant antigen encoded by a gene which is a member of a multi-gene family. With the recent availability of two <jats:italic>E<\/jats:italic>. <jats:italic>granulosus<\/jats:italic> draft genomes, we sought to map the <jats:italic>eg95<\/jats:italic> gene family to the genomes. We were unable to map unequivocally any of the <jats:italic>eg95<\/jats:italic> gene family members which had previously been characterized by cloning and sequencing both strands of genomic DNA fragments. Our inability to map EG95-related genes to the genomes has revealed limitations in the assembled sequence data when utilized for gene family analyses. This study contrasts with the expectations expressed in often high-profile publications describing draft genomes of parasitic organisms, highlighting deficiencies in currently available genomic resources for <jats:italic>E<\/jats:italic>. <jats:italic>granulosus<\/jats:italic> and provides a cautionary note for research which seeks to utilize these genome datasets.<\/jats:p>","DOI":"10.1017\/s0031182017001767","type":"journal-article","created":{"date-parts":[[2017,11,27]],"date-time":"2017-11-27T15:43:16Z","timestamp":1511797396000},"page":"807-813","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":9,"title":["Limitations of the <i>Echinococcus granulosus<\/i> genome sequence assemblies for analysis of the gene family encoding the EG95 vaccine antigen"],"prefix":"10.1017","volume":"145","author":[{"given":"Charles 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