{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T17:02:47Z","timestamp":1774976567140,"version":"3.50.1"},"reference-count":14,"publisher":"Cambridge University Press (CUP)","issue":"7","license":[{"start":{"date-parts":[[2017,11,23]],"date-time":"2017-11-23T00:00:00Z","timestamp":1511395200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["www.cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Trypanosoma equiperdum<\/jats:italic> is a protozoan parasite responsible for causing Dourine, a debilitating neglected veterinary disease, found worldwide affecting equids. It is the only pathogenic trypanosome species that does not require an invertebrate vector for transmission, thus being passed from animal to animal <jats:italic>via<\/jats:italic> coitus. At present, there is no officially recognized form of chemotherapeutic treatment and therefore all confirmed (or suspected) cases of infected animals must be slaughtered immediately. For many global communities and farming populations, which rely heavily on their animals for their livelihood, such stringent regulations can seriously enhance the socio-economic problems attributing to poverty. Two reference drugs, together with 37 novel diamidine compounds were tested <jats:italic>in vitro<\/jats:italic> using a 72 h drug sensitivity assay to determine their efficacy against two axenically adapted <jats:italic>T. equiperdum<\/jats:italic> strains. Further <jats:italic>in vivo<\/jats:italic> investigations in mouse models of infection against 4 \u2018true\u2019 <jats:italic>T. equiperdum<\/jats:italic> strains were performed using the 17 most active diamidines. Single bolus doses of 10 mg kg<jats:sup>\u22121<\/jats:sup>, given i.p. were administered to NMRI mice infected with one of the 4 <jats:italic>T. equiperdum<\/jats:italic> strains. The results obtained from this study show that experimentally <jats:italic>T. equiperdum<\/jats:italic> can indeed be effectively treated with chemotherapy using <jats:italic>in vivo<\/jats:italic> mouse models of infection.<\/jats:p>","DOI":"10.1017\/s0031182017002098","type":"journal-article","created":{"date-parts":[[2017,11,23]],"date-time":"2017-11-23T03:36:07Z","timestamp":1511408167000},"page":"953-960","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":4,"title":["<i>In vitro<\/i> and <i>in vivo<\/i> efficacy of diamidines against <i>Trypanosoma equiperdum<\/i> strains"],"prefix":"10.1017","volume":"145","author":[{"given":"Kirsten","family":"Gillingwater","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2017,11,23]]},"reference":[{"key":"S0031182017002098_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2005.05.010"},{"key":"S0031182017002098_ref5","unstructured":"Gillingwater K (2007) Discovery of novel active diamidines as clinical candidates against Trypanosoma evansi infection. PhD theses, University of Basel, Basel, Switzerland."},{"key":"S0031182017002098_ref14","doi-asserted-by":"publisher","DOI":"10.2174\/1568011054222319"},{"key":"S0031182017002098_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.01.034"},{"key":"S0031182017002098_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2007.05.020"},{"key":"S0031182017002098_ref9","volume-title":"The Trypanosomes of Mammals. A Zoological Monograph","author":"Hoare","year":"1972"},{"key":"S0031182017002098_ref4","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evx102"},{"key":"S0031182017002098_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2012.12.006"},{"key":"S0031182017002098_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(98)00146-0"},{"key":"S0031182017002098_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(03)00160-2"},{"key":"S0031182017002098_ref7","first-page":"649","article-title":"Seroepidemiological survey of Trypanozoon infection in horses in the suspected dourine-infected Bale highlands of the Oromia region, Ethiopia","volume":"29","author":"Hagos","year":"2010a","journal-title":"Revue Scientifique et Technique\/Office International des Epizooties"},{"key":"S0031182017002098_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.03.041"},{"key":"S0031182017002098_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(97)00079-X"},{"key":"S0031182017002098_ref13","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-016-1755-3"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182017002098","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,14]],"date-time":"2019-04-14T15:57:09Z","timestamp":1555257429000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182017002098\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,23]]},"references-count":14,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["S0031182017002098"],"URL":"https:\/\/doi.org\/10.1017\/s0031182017002098","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,23]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2017\u00a0","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}