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This review briefly describes the history of CRISPR\/Cas9 and the principles behind its use along with documenting its implementation in apicomplexan parasites, especially <jats:italic>Plasmodium<\/jats:italic> spp. and <jats:italic>Toxoplasma gondii<\/jats:italic>. We also discuss the recent use of CRISPR\/Cas9 for whole genome screening of gene knockout mutants in <jats:italic>T. gondii<\/jats:italic> and highlight its use for seminal genetic manipulations of <jats:italic>Cryptosporidium<\/jats:italic> spp. Finally, we consider new variations of CRISPR\/Cas9 that have yet to be implemented in apicomplexans. Whereas CRISPR\/Cas9 has already accelerated rapid interrogation of gene function in apicomplexans, the full potential of this technology is yet to be realized as new variations and innovations are integrated into the field.<\/jats:p>","DOI":"10.1017\/s003118201800001x","type":"journal-article","created":{"date-parts":[[2018,2,21]],"date-time":"2018-02-21T09:14:10Z","timestamp":1519204450000},"page":"1119-1126","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":41,"title":["New and emerging uses of CRISPR\/Cas9 to genetically manipulate apicomplexan parasites"],"prefix":"10.1017","volume":"145","author":[{"given":"Manlio","family":"Di Cristina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vern 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