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Genomic DNA from trophozoites was tested by PCR-sequence analysis at three loci (<jats:italic>\u03b2<\/jats:italic>-giardin, glutamate dehydrogenase and triose phosphate isomerase). Sequence analysis showed assemblages A and B in 41 (87.2%) and six (12.8%) isolates, respectively. Two of the 41 assemblage A samples were genotyped as sub-assemblage AI, and 39 were genotyped as sub-assemblage AII. Four previously identified multilocus genotypes (MLGs: AI-1, AII-1, AII-4 and AII-9) and six likely novel variations of MLGs were found. In agreement with previous studies, sequences from assemblage B isolates were characterized by a large genetic variability and by the presence of heterogeneous positions, which prevent the definition of MLGs. This study also investigated whether there was a relationship between the assemblage and clinical data (including drug resistance). However, due to the large number of genotypes and the relatively small number of samples, no significant associations with the clinical data were found.<\/jats:p>","DOI":"10.1017\/s0031182018000409","type":"journal-article","created":{"date-parts":[[2018,3,20]],"date-time":"2018-03-20T02:45:34Z","timestamp":1521513934000},"page":"1577-1587","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":27,"title":["The first multilocus genotype analysis of <i>Giardia intestinalis<\/i> in humans in the Czech 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