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With an aim to identify target antigens for sensitive serodiagnosis, adenylate kinase 3 of <jats:italic>C. sinensis<\/jats:italic> (CsAK3) was successfully expressed in soluble form in <jats:italic>Escherichia coli<\/jats:italic> by fusion to an RNA-interacting domain derived from human Lys-tRNA synthetase and purified by Ni<jats:sup>2+<\/jats:sup>-affinity chromatography. Anti-CsAK3 serum was raised by immunization of mice, and Western blotting confirmed that CsAK3 was expressed in adult-stage <jats:italic>C. sinensis<\/jats:italic>. Histochemical analysis showed that CsAK3 was localized to the subtegumental tissue of <jats:italic>C. sinensis<\/jats:italic> and was excreted into the bile duct of the host. When tested against sera from various parasite-infected patients by enzyme-linked immunosorbent assay, the recombinant CsAK3 elicited a specific response to <jats:italic>C. sinensis<\/jats:italic>-infected sera. The results suggest that CsAK3, either alone or in combination with other antigens, could be used for improving the clinical diagnosis of clonorchiasis.<\/jats:p>","DOI":"10.1017\/s0031182018000434","type":"journal-article","created":{"date-parts":[[2018,3,27]],"date-time":"2018-03-27T07:41:42Z","timestamp":1522136502000},"page":"1531-1539","update-policy":"http:\/\/dx.doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":9,"title":["Recombinant adenylate kinase 3 from liver fluke <i>Clonorchis sinensis<\/i> for histochemical analysis and serodiagnosis of clonorchiasis"],"prefix":"10.1017","volume":"145","author":[{"given":"Soon Bin","family":"Kwon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hae Sun","family":"Woo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tae Yun","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju Yeong","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye Min","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun Soo","family":"Jang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun-Min","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tai-Soon","family":"Yong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baik Lin","family":"Seong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2018,3,27]]},"reference":[{"key":"S0031182018000434_ref11","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn180"},{"key":"S0031182018000434_ref2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-014-3820-1"},{"key":"S0031182018000434_ref42","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2168.2002.02143.x"},{"key":"S0031182018000434_ref40","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(02)02169-2"},{"key":"S0031182018000434_ref43","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-005-1305-y"},{"key":"S0031182018000434_ref15","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109759200"},{"key":"S0031182018000434_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.03.016"},{"key":"S0031182018000434_ref32","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-013-2508-4"},{"key":"S0031182018000434_ref28","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.1993.31.1.21"},{"key":"S0031182018000434_ref33","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-013-3330-6"},{"key":"S0031182018000434_ref45","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001208"},{"key":"S0031182018000434_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.11.176"},{"key":"S0031182018000434_ref12","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-10-8"},{"key":"S0031182018000434_ref26","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2017.1311455"},{"key":"S0031182018000434_ref14","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw291"},{"key":"S0031182018000434_ref6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002677"},{"key":"S0031182018000434_ref10","doi-asserted-by":"publisher","DOI":"10.3390\/ijms130810368"},{"key":"S0031182018000434_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2005.11.040"},{"key":"S0031182018000434_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2013.10.035"},{"key":"S0031182018000434_ref13","doi-asserted-by":"publisher","DOI":"10.1021\/bi0006722"},{"key":"S0031182018000434_ref29","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2004.42.4.169"},{"key":"S0031182018000434_ref41","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.01.039"},{"key":"S0031182018000434_ref30","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-2179-1"},{"key":"S0031182018000434_ref5","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2003.41.1.35"},{"key":"S0031182018000434_ref27","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.1998.36.1.37"},{"key":"S0031182018000434_ref23","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm256"},{"key":"S0031182018000434_ref22","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti1014"},{"key":"S0031182018000434_ref48","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-013-3331-5"},{"key":"S0031182018000434_ref3","unstructured":"Cho S , Chung B and Lee S (2013) National survey of intestinal parasitic infections in Korea, 8th Report 2013. 2005-811X. 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