{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T02:49:52Z","timestamp":1779331792648,"version":"3.51.4"},"reference-count":41,"publisher":"Cambridge University Press (CUP)","issue":"14","license":[{"start":{"date-parts":[[2018,5,28]],"date-time":"2018-05-28T00:00:00Z","timestamp":1527465600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Oligosaccharides are broadly present on<jats:italic>Leishmania<\/jats:italic>cell surfaces. They can be useful for the leishmaniases diagnosis and also helpful in identifying new cell markers for the disease. The disaccharide Gal<jats:italic>\u03b1<\/jats:italic>1-3Gal<jats:italic>\u03b2<\/jats:italic>is the immunodominant saccharide in<jats:italic>Leishmania<\/jats:italic>cell surface and is the unique non-reducing terminal glycosphingolipids structure recognized by anti-<jats:italic>\u03b1<\/jats:italic>-Gal. This study describes an enzyme-linked immunosorbent assay (ELISA) used to measure serum levels of anti-<jats:italic>\u03b1<\/jats:italic>-galactosyl (<jats:italic>\u03b1<\/jats:italic>-Gal) antibodies in patients with cutaneous leishmaniasis (CL). Optimal ELISA conditions were established and two neoglycoproteins (NGP) containing the Gal\u03b11-3Gal terminal fraction (Gal\u03b11-3Gal\u03b21-4GlcNAc-HAS and Gal\u03b11-3Gal-HAS) and one Gal\u03b11-3Gal NGP analogue (Gal\u03b11-3Gal\u03b21-3GlcNAc-HAS) were used as antigens. Means of anti-<jats:italic>\u03b1<\/jats:italic>-Gal antibody titres of CL patients were significantly higher (<jats:italic>P<\/jats:italic>&lt; 0.05) than the healthy individuals for all NGPs tested. Sensitivity and specificity of all NGPs ranged from 62.2 to 78.4% and 58.3 to 96.7%, respectively. In conclusion, the NGPs can be used for CL diagnosis.<\/jats:p>","DOI":"10.1017\/s0031182018000720","type":"journal-article","created":{"date-parts":[[2018,5,28]],"date-time":"2018-05-28T10:21:26Z","timestamp":1527502886000},"page":"1938-1948","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":5,"title":["Analysis of<i>Leishmania<\/i>mimetic neoglycoproteins for the cutaneous leishmaniasis diagnosis"],"prefix":"10.1017","volume":"145","author":[{"given":"L\u00edgia Moraes Barizon","family":"de Souza","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanete","family":"Thomaz Soccol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo Rasmussen","family":"Petterle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle 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