{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T04:45:32Z","timestamp":1776401132228,"version":"3.51.2"},"reference-count":61,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2018,7,17]],"date-time":"2018-07-17T00:00:00Z","timestamp":1531785600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fecal samples from wild-caught common voles (<jats:italic>n<\/jats:italic>= 328) from 16 locations in the Czech Republic were screened for<jats:italic>Cryptosporidium<\/jats:italic>by microscopy and PCR\/sequencing at loci coding small-subunit rRNA,<jats:italic>Cryptosporidium<\/jats:italic>oocyst wall protein, actin and 70 kDa heat shock protein.<jats:italic>Cryptosporidium<\/jats:italic>infections were detected in 74 voles (22.6%). Rates of infection did not differ between males and females nor between juveniles and adults. Phylogenetic analysis revealed the presence of eight<jats:italic>Cryptosporidium<\/jats:italic>species\/genotypes including two new species,<jats:italic>C. alticolis<\/jats:italic>and<jats:italic>C. microti<\/jats:italic>. These species from wild-caught common voles were able to infect common and meadow voles under experimental conditions, with a prepatent period of 3\u20135 days post-infection (DPI), but they were not infectious for various other rodents or chickens. Meadow voles lost infection earlier than common voles (11\u201314<jats:italic>vs<\/jats:italic>13\u201316 DPI) and had significantly lower infection intensity.<jats:italic>Cryptosporidium alticolis<\/jats:italic>infects the anterior small intestine and has larger oocysts (5.4 \u00d7 4.9<jats:italic>\u00b5<\/jats:italic>m), whereas<jats:italic>C. microti<\/jats:italic>infects the large intestine and has smaller oocysts (4.3 \u00d7 4.1<jats:italic>\u00b5<\/jats:italic>m). None of the rodents developed clinical signs of infection. Genetic and biological data support the establishment of<jats:italic>C. alticolis<\/jats:italic>and<jats:italic>C. microti<\/jats:italic>as separate species of the genus<jats:italic>Cryptosporidium<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0031182018001142","type":"journal-article","created":{"date-parts":[[2018,7,17]],"date-time":"2018-07-17T07:25:52Z","timestamp":1531812352000},"page":"220-233","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":40,"title":["Diversity of<i>Cryptosporidium<\/i>in common voles and description of<i>Cryptosporidium alticolis<\/i>sp. n. and<i>Cryptosporidium microti<\/i>sp. n. (Apicomplexa: Cryptosporidiidae)"],"prefix":"10.1017","volume":"146","author":[{"given":"Michaela","family":"Hor\u010di\u010dkov\u00e1","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0160\u00e1rka","family":"\u010condlov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikola","family":"Holubov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bohumil","family":"Sak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dana","family":"Kv\u011bto\u0148ov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lenka","family":"Hl\u00e1skov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roman","family":"Kone\u010dn\u00fd","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franti\u0161ek","family":"Sedl\u00e1\u010dek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Clark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Giddings","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6843-5551","authenticated-orcid":false,"given":"John","family":"McEvoy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0013-6090","authenticated-orcid":false,"given":"Martin","family":"Kv\u00e1\u010d","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2018,7,17]]},"reference":[{"key":"S0031182018001142_ref57","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.1128\/AEM.65.4.1578-1583.1999","article-title":"Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus","volume":"65","author":"Xiao","year":"1999","journal-title":"Applied and Environmental Microbiology"},{"key":"S0031182018001142_ref6","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0276-y"},{"key":"S0031182018001142_ref45","first-page":"59","article-title":"Occurrence of Cryptosporidium parvum infection in wild small mammals in District of Mazury Lake (Poland)","volume":"38","author":"Sinski","year":"1993","journal-title":"Acta Parasitologica"},{"key":"S0031182018001142_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2008.11.005"},{"key":"S0031182018001142_ref33","first-page":"50","article-title":"Differential staining of cryptosporidia by aniline-carbol-methyl violet and tartrazine in smears from feces and scrapings of intestinal mucosa","volume":"32","author":"Mil\u00e1\u010dek","year":"1985","journal-title":"Folia Parasitologica"},{"key":"S0031182018001142_ref51","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(98)00226-X"},{"key":"S0031182018001142_ref35","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.08.029"},{"key":"S0031182018001142_ref31","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01386-09"},{"key":"S0031182018001142_ref43","doi-asserted-by":"publisher","DOI":"10.1645\/GE-202R1"},{"key":"S0031182018001142_ref61","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-43.4.586"},{"key":"S0031182018001142_ref56","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.2006.00950.x"},{"key":"S0031182018001142_ref53","doi-asserted-by":"publisher","DOI":"10.1645\/0022-3395(2002)088[0388:MPAERO]2.0.CO;2"},{"key":"S0031182018001142_ref52","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.6.2385-2391.2000"},{"key":"S0031182018001142_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2015.10.179"},{"key":"S0031182018001142_ref60","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.12.7574-7577.2004"},{"key":"S0031182018001142_ref37","first-page":"237","volume-title":"Cryptosporidium: Parasite and Disease","author":"Nichols","year":"2014"},{"key":"S0031182018001142_ref15","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01034-07"},{"key":"S0031182018001142_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2004.04.005"},{"key":"S0031182018001142_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2013.01.011"},{"key":"S0031182018001142_ref25","doi-asserted-by":"publisher","DOI":"10.14411\/fp.2008.012"},{"key":"S0031182018001142_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.02.023"},{"key":"S0031182018001142_ref44","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.07.039"},{"key":"S0031182018001142_ref49","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2015.10.002"},{"key":"S0031182018001142_ref42","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-7091-1562-6_1"},{"key":"S0031182018001142_ref9","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050283"},{"key":"S0031182018001142_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejop.2017.12.006"},{"key":"S0031182018001142_ref55","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(00)00331-9"},{"key":"S0031182018001142_ref46","first-page":"173","article-title":"The role of wild rodents in ecology of cryptosporidiosis in Poland","volume":"45","author":"Sinski","year":"1998","journal-title":"Folia Parasitologica"},{"key":"S0031182018001142_ref17","doi-asserted-by":"publisher","DOI":"10.1080\/10635150390235520"},{"key":"S0031182018001142_ref62","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2007.03.024"},{"key":"S0031182018001142_ref34","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-012-2821-1"},{"key":"S0031182018001142_ref59","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.1.72-97.2004"},{"key":"S0031182018001142_ref39","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.3.1154-1162.2001"},{"key":"S0031182018001142_ref41","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02346-12"},{"key":"S0031182018001142_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2006.08.031"},{"key":"S0031182018001142_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.03.005"},{"key":"S0031182018001142_ref47","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(97)00115-8"},{"key":"S0031182018001142_ref22","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.8.4446-4454.2005"},{"key":"S0031182018001142_ref20","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-005-1459-7"},{"key":"S0031182018001142_ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1996.tb05015.x"},{"key":"S0031182018001142_ref2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-008-1179-x"},{"key":"S0031182018001142_ref29","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-30.1.110"},{"key":"S0031182018001142_ref4","doi-asserted-by":"publisher","DOI":"10.1645\/GE-3096RN"},{"key":"S0031182018001142_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(14)70772-8"},{"key":"S0031182018001142_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2017.04.013"},{"key":"S0031182018001142_ref23","doi-asserted-by":"publisher","DOI":"10.14411\/fp.2008.011"},{"key":"S0031182018001142_ref54","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"S0031182018001142_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.04.007"},{"key":"S0031182018001142_ref27","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-7091-1562-6_5"},{"key":"S0031182018001142_ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejop.2018.02.001"},{"key":"S0031182018001142_ref30","doi-asserted-by":"publisher","DOI":"10.3201\/eid2002.121797"},{"key":"S0031182018001142_ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2014.03.030"},{"key":"S0031182018001142_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.11.010"},{"key":"S0031182018001142_ref38","doi-asserted-by":"publisher","DOI":"10.5604\/12321966.1141359"},{"key":"S0031182018001142_ref19","first-page":"95","article-title":"Bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symposium Series"},{"key":"S0031182018001142_ref50","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182017001524"},{"key":"S0031182018001142_ref40","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2015.07.002"},{"key":"S0031182018001142_ref7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7998.1998.tb00013.x"},{"key":"S0031182018001142_ref16","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00848-07"},{"key":"S0031182018001142_ref48","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2015.03.003"},{"key":"S0031182018001142_ref3","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182002001865"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182018001142","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,6]],"date-time":"2020-11-06T02:17:06Z","timestamp":1604629026000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182018001142\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,17]]},"references-count":61,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["S0031182018001142"],"URL":"https:\/\/doi.org\/10.1017\/s0031182018001142","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,17]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2018\u00a0","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}