{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:10:55Z","timestamp":1788567055680,"version":"build-2803163510"},"reference-count":45,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2018,11,14]],"date-time":"2018-11-14T00:00:00Z","timestamp":1542153600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study was undertaken to investigate the antiparasitic activity of extracellular products of <jats:italic>Streptomyces albus<\/jats:italic>. Bioactivity-guided isolation of chloroform extracts affording a compound showing potent activity. The structure of the compound was elucidated as salinomycin (SAL) by EI-MS, <jats:sup>1<\/jats:sup>H NMR and <jats:sup>13<\/jats:sup>C NMR. <jats:italic>In vitro<\/jats:italic> test showed that SAL has potent anti-parasitic efficacy against theronts of <jats:italic>Ichthyophthirius multifiliis<\/jats:italic> with 10 min, 1, 2, 3 and 4 h (effective concentration) EC<jats:sub>50<\/jats:sub> (95% confidence intervals) of 2.12 (2.22\u20132.02), 1.93 (1.98\u20131.88), 1.42 (1.47\u20131.37), 1.35 (1.41\u20131.31) and 1.11 (1.21\u20131.01) mg L<jats:sup>\u22121<\/jats:sup>. <jats:italic>In vitro<\/jats:italic> antiparasitic assays revealed that SAL could be 100% effective against <jats:italic>I. multifiliis<\/jats:italic> encysted tomonts at a concentration of 8.0 mg L<jats:sup>\u22121<\/jats:sup>. <jats:italic>In vivo<\/jats:italic> test demonstrated that the number of <jats:italic>I. multifiliis<\/jats:italic> trophonts on <jats:italic>Erythroculter ilishaeformis<\/jats:italic> treated with SAL was markedly lower than that of control group at 10 days after exposed to theronts (<jats:italic>P<\/jats:italic> &lt; 0.05). In the control group, 80% mortality was observed owing to heavy <jats:italic>I. multifiliis<\/jats:italic> infection at 10 days. On the other hand, only 30.0% mortality was recorded in the group treated with 8.0 mg L<jats:sup>\u22121<\/jats:sup> SAL. The median lethal dose (LD<jats:sub>50<\/jats:sub>) of SAL for <jats:italic>E. ilishaeformis<\/jats:italic> was 32.9 mg L<jats:sup>\u22121<\/jats:sup>.<\/jats:p>","DOI":"10.1017\/s0031182018001919","type":"journal-article","created":{"date-parts":[[2018,11,14]],"date-time":"2018-11-14T08:34:49Z","timestamp":1542184489000},"page":"521-526","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":11,"title":["Evaluation of salinomycin isolated from <i>Streptomyces albus<\/i> JSY-2 against the ciliate, <i>Ichthyophthirius multifiliis<\/i>"],"prefix":"10.1017","volume":"146","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6134-1018","authenticated-orcid":false,"given":"Jia-Yun","family":"Yao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Yue","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Yi","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Xia","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Lin","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gui-Lian","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-Bin","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Feng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Yu","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Min","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2018,11,14]]},"reference":[{"key":"S0031182018001919_ref43","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2011.05.030"},{"key":"S0031182018001919_ref42","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-012-3022-7"},{"key":"S0031182018001919_ref40","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-014-4020-8"},{"key":"S0031182018001919_ref39","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.07.021"},{"key":"S0031182018001919_ref37","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-008-1044-y"},{"key":"S0031182018001919_ref36","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-012-2893-y"},{"key":"S0031182018001919_ref35","doi-asserted-by":"publisher","DOI":"10.1007\/s002030100345"},{"key":"S0031182018001919_ref34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0426.1993.tb00400.x"},{"key":"S0031182018001919_ref33","doi-asserted-by":"publisher","DOI":"10.1577\/1548-8454(2001)063<0293:TFIIIC>2.0.CO;2"},{"key":"S0031182018001919_ref30","doi-asserted-by":"publisher","DOI":"10.1577\/1548-8667(2002)014<0145:EOPPIT>2.0.CO;2"},{"key":"S0031182018001919_ref21","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000051428"},{"key":"S0031182018001919_ref20","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000055529"},{"key":"S0031182018001919_ref16","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1046\/j.1439-0426.2001.00279.x","article-title":"Temperature and salinity dependent development of a Nordic strain of Ichthyophthirius multifiliis from rainbow trout","volume":"17","author":"Li","year":"2001","journal-title":"Journal of Applied Ichthyology"},{"key":"S0031182018001919_ref15","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005419617371"},{"key":"S0031182018001919_ref14","unstructured":"Klinger R and Floyd RF (2002) Introduction to freshwater fish parasites. 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