{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T11:45:50Z","timestamp":1789904750563,"version":"4.0.1"},"reference-count":38,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[2019,1,14]],"date-time":"2019-01-14T00:00:00Z","timestamp":1547424000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Amoebae are single-celled parasites frequently colonizing human gut. However, few molecular tools are available for accurate identification. Here, we evaluated a panel of polymerase chain reactions (PCRs) targeting <jats:italic>Entamoeba histolytica<\/jats:italic>, <jats:italic>Entamoeba dispar<\/jats:italic>, <jats:italic>Entamoeba coli<\/jats:italic>, <jats:italic>Entamoeba hartmanni<\/jats:italic>, <jats:italic>Entamoeba polecki<\/jats:italic>, <jats:italic>Endolimax nana<\/jats:italic> and <jats:italic>Iodamoeba b\u00fctschlii<\/jats:italic>. Thirty-six faecal samples (18 containing at least one amoeba species by microscopy and 18 microscopy negative for amoebae) were tested. Real-time PCRs were used for detection and differentiation of <jats:italic>E. histolytica<\/jats:italic> and <jats:italic>E. dispar<\/jats:italic>. Conventional PCR with Sanger sequencing were applied for detection and differentiation of <jats:italic>E. coli<\/jats:italic>, <jats:italic>E. hartmanni<\/jats:italic>, <jats:italic>E. polecki<\/jats:italic>, <jats:italic>E. nana<\/jats:italic> and <jats:italic>I. b\u00fctschlii.<\/jats:italic> All microscopy results were confirmed by DNA-based methods. However, more samples were positive for single and mixed amoebic species by DNA-based assays than by microscopy (22 <jats:italic>vs<\/jats:italic> 18 and 7 <jats:italic>vs<\/jats:italic> 1, respectively). DNA sequencing allowed identification of <jats:italic>E. coli<\/jats:italic> subtypes (ST1 and ST2), showed low intra-specific variation within <jats:italic>E. hartmanni<\/jats:italic>, identified two phylogenetically distinct groups within <jats:italic>E. nana<\/jats:italic>, and identified <jats:italic>Iodamoeba<\/jats:italic> at the ribosomal lineage level. Taking into account the high intra-genetic diversity within some of the species at the small subunit (SSU) rRNA gene level, amplification of SSU rRNA genes with subsequent sequencing represents a useful method for detecting, differentiating and subtyping intestinal amoebae.<\/jats:p>","DOI":"10.1017\/s0031182018002196","type":"journal-article","created":{"date-parts":[[2019,1,14]],"date-time":"2019-01-14T04:07:49Z","timestamp":1547438869000},"page":"821-827","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":11,"title":["Development and evaluation of molecular tools for detecting and differentiating intestinal amoebae in healthy individuals"],"prefix":"10.1017","volume":"146","author":[{"given":"Amal","family":"Chihi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1417-7048","authenticated-orcid":false,"given":"Christen 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