{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T12:34:47Z","timestamp":1763037287228,"version":"3.40.5"},"reference-count":25,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T00:00:00Z","timestamp":1552348800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bovine trichomoniasis is a notifiable, reproductive disease of cattle caused by the parasite <jats:italic>Tritrichomonas foetus.<\/jats:italic> Culturing with modified Diamond's medium (MDM) is required to increase the low number of organisms received from a preputial sample, but is limited in application to remote areas as it requires continuous cold chain storage. This study utilized lyophilization to sustain the viability of MDM during transport in lieu of a continuous cold chain. All lyophilized MDM was able to sustain <jats:italic>T. foetus<\/jats:italic> after storage for 42 days at 24 \u00b0C, and the results demonstrated that lyophilized MDM was equally as viable as refrigerated liquid MDM. Storage of lyophilized MDM at room temperature for 1 and 7 days did not impact <jats:italic>T. foetus<\/jats:italic> yield, both with and without exposure to light. A limitation of the lyophilized MDM was demonstrated with a significant decrease in <jats:italic>T. foetus<\/jats:italic> yield when the media was stored at 37 and 58 \u00b0C. The lyophilization of MDM provides a robust method of transporting and storing medium prior to reconstitution and inoculation, for use in <jats:italic>T. foetus<\/jats:italic> diagnosis and surveillance in remote areas.<\/jats:p>","DOI":"10.1017\/s0031182019000258","type":"journal-article","created":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T06:16:01Z","timestamp":1552371361000},"page":"1184-1187","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":1,"title":["Addressing the constraints of <i>Tritrichomonas foetus<\/i> sample collection in remote areas: lyophilized modified Diamond's media as a substitute for liquid medium"],"prefix":"10.1017","volume":"146","author":[{"given":"Gemma","family":"Rush","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6592-7232","authenticated-orcid":false,"given":"Michael William","family":"Reynolds","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8787-6755","authenticated-orcid":false,"given":"Nichola Eliza Davies","family":"Calvani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1459-9117","authenticated-orcid":false,"given":"Jan","family":"\u0160lapeta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2019,3,12]]},"reference":[{"doi-asserted-by":"publisher","key":"S0031182019000258_ref25","DOI":"10.1023\/B:PHAM.0000016234.73023.75"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref24","DOI":"10.1016\/j.ijpara.2012.10.004"},{"unstructured":"OIE (2018) OIE-Listed diseases, infections and infestations in force in 2018. Retrieved from OIE: http:\/\/www.oie.int\/en\/animal-health-in-the-world\/oie-listed-diseases-2018\/ (accessed 20 August 2018).","key":"S0031182019000258_ref21"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref20","DOI":"10.1007\/s11626-017-0184-8"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref18","DOI":"10.1016\/S0093-691X(03)00138-9"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref16","DOI":"10.1111\/j.1751-0813.1988.tb14445.x"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref14","DOI":"10.1016\/S0304-4017(00)00199-0"},{"volume-title":"Protozoan Parasites of Domestic Animals and of Man","year":"1973","author":"Levine","key":"S0031182019000258_ref13"},{"volume-title":"In Vitro Cultivation of Protozoan Parasites","year":"1983","author":"Jensen","key":"S0031182019000258_ref12"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref11","DOI":"10.1016\/j.ejpb.2009.02.002"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref8","DOI":"10.1016\/j.ejpb.2018.02.003"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref6","DOI":"10.1016\/j.theriogenology.2007.06.019"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref4","DOI":"10.1016\/j.ejps.2018.07.016"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref1","DOI":"10.1007\/s00436-018-6029-x"},{"volume-title":"Bovine Trichomoniasis: Australian and New Zealand Standard Diagnostic Procedure","year":"2013","author":"Buller","key":"S0031182019000258_ref5"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref2","DOI":"10.1016\/j.vetpar.2013.12.029"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref7","DOI":"10.1016\/j.tvjl.2014.02.003"},{"unstructured":"Biomed Diagnostics (n.d.) InPouch\u2122 TF (Tritrichomonas foetus Bovine). Retrieved from Biomed Diagnostics: https:\/\/biomeddiagnostics.com\/resources\/files\/InPouch%20TF%20Bovine%20PDS%20V4_2.pdf (accessed 28 August 2018).","key":"S0031182019000258_ref3"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref10","DOI":"10.1177\/1098612X17693499"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref17","DOI":"10.1016\/j.vetpar.2015.08.010"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref23","DOI":"10.1016\/j.exppara.2010.04.024"},{"unstructured":"Eccles J (2016) Trichomoniasis in the Northern Territory: AgNote. Retrieved from Northern Territory Government: https:\/\/www.nt.gov.au\/__data\/assets\/pdf_file\/0016\/233116\/746.pdf (accessed 10 September 2018).","key":"S0031182019000258_ref9"},{"unstructured":"OIE Terrestrial Manual (2012) Trichomonosis. Retrieved from OIE Terrestrial Manual 2012, Chapter 2.4.16: http:\/\/www.oie.int\/fileadmin\/Home\/eng\/Health_standards\/tahm\/2.04.16_TRICHOMONOSIS.pdf (accessed 20 August 2018).","key":"S0031182019000258_ref22"},{"doi-asserted-by":"publisher","key":"S0031182019000258_ref15","DOI":"10.1016\/j.cryobiol.2006.03.011"},{"unstructured":"National Farmer's Federation (2017) Food, Fibre and Forestry Facts: A Summary of Australia's Agricultural Sector. Retrieved from National Farmer's Federation, Australia: http:\/\/www.nff.org.au\/farm-facts.html (accessed 2 September 2018).","key":"S0031182019000258_ref19"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182019000258","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,5]],"date-time":"2019-08-05T04:50:44Z","timestamp":1564980644000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182019000258\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,12]]},"references-count":25,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2019,8]]}},"alternative-id":["S0031182019000258"],"URL":"https:\/\/doi.org\/10.1017\/s0031182019000258","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"type":"print","value":"0031-1820"},{"type":"electronic","value":"1469-8161"}],"subject":[],"published":{"date-parts":[[2019,3,12]]},"assertion":[{"value":"Copyright \u00a9 Cambridge University Press 2019\u00a0","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}