{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T23:48:32Z","timestamp":1793058512792,"version":"4.5.0"},"reference-count":28,"publisher":"Cambridge University Press (CUP)","issue":"10","license":[{"start":{"date-parts":[[2019,6,24]],"date-time":"2019-06-24T00:00:00Z","timestamp":1561334400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Fasciola jacksoni<\/jats:italic> is a significant contributor to the health and mortality of Asian elephants, particularly those in Sri Lanka. Despite the impact of fascioliasis on elephant populations, it is a neglected veterinary disease with limited taxonomic understanding. Molecular characterization and phylogenetic analysis of <jats:italic>F. jacksoni<\/jats:italic> were carried out to evaluate its suggested basal position in the Fasciolidae. Adult worms were collected during post-mortem of elephants, and eggs were collected from living elephants in National parks across Sri Lanka. Using the mitochondrial genes nicotinamide dehydrogenase subunit 1 (<jats:italic>nad<\/jats:italic>1) and cytochrome oxidase subunit 1 (<jats:italic>cox<\/jats:italic>1), and a partial 28S ribosomal DNA (28S rDNA), DNA sequences were generated from the <jats:italic>F. jacksoni<\/jats:italic> adult and egg material. Maximum likelihood (ML) phylogenetic analyses did not resolve <jats:italic>F. jacksoni<\/jats:italic> to be basal to the Fasciolidae. Furthermore, the ML analyses showed that the genus <jats:italic>Fasciola<\/jats:italic> was not monophyletic and that <jats:italic>F. jacksoni<\/jats:italic> was a sister species to the deer liver fluke <jats:italic>Fascioloides magna.<\/jats:italic> A clear framework is required to determine the taxonomic status of <jats:italic>F. jacksoni<\/jats:italic> and this current study provides the first detailed application of molecular techniques from multiple hosts across Sri Lanka with the production of reference DNA sequences for this important parasite.<\/jats:p>","DOI":"10.1017\/s0031182019000519","type":"journal-article","created":{"date-parts":[[2019,5,14]],"date-time":"2019-05-14T07:49:14Z","timestamp":1557820154000},"page":"1247-1255","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":14,"title":["Molecular characterization of <i>Fasciola jacksoni<\/i> from wild elephants (<i>Elephas maximus maximus<\/i>) of Sri Lanka: a taxonomic evaluation"],"prefix":"10.1017","volume":"146","author":[{"given":"R. P. V. J.","family":"Rajapakse","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4055-6524","authenticated-orcid":false,"given":"S. P.","family":"Lawton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. J. K.","family":"Karunathilake","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. V. P.","family":"Perera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. T. B.","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. H.","family":"Le","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2019,6,24]]},"reference":[{"key":"S0031182019000519_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2015.09.024"},{"key":"S0031182019000519_ref34","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1935(17)34691-2"},{"key":"S0031182019000519_ref33","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2012.12.015"},{"key":"S0031182019000519_ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2015.11.001"},{"key":"S0031182019000519_ref30","unstructured":"Perera BVP , Rajapakse RPVJ , Silva-fletcher A , Thewarage LD and Jinadasa HRN (2015) Emerging parasitic and infectious diseases of wild Asian Elephants (Elephas Maximus) in Udawalawe National Park, Sri Lanka. American Association of Zoo Veterinarians. Oregon, USA."},{"key":"S0031182019000519_ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.09.001"},{"key":"S0031182019000519_ref22","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2008.79.248"},{"key":"S0031182019000519_ref20","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-7-150"},{"key":"S0031182019000519_ref17","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-017-1968-0"},{"key":"S0031182019000519_ref13","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msw054"},{"key":"S0031182019000519_ref11","first-page":"109","article-title":"Studies on some aspects of fascioliasis in Asian elephants (Elephas maximus)","volume":"11","author":"Islam","year":"1997","journal-title":"Journal of Veterinary Parasitology"},{"key":"S0031182019000519_ref8","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-013-3326-2"},{"key":"S0031182019000519_ref5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1096-3642.1882.tb00668.x"},{"key":"S0031182019000519_ref4","unstructured":"Cobbold T. 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