{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:42:49Z","timestamp":1791895369928,"version":"4.3.4"},"reference-count":53,"publisher":"Cambridge University Press (CUP)","issue":"11","license":[{"start":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T00:00:00Z","timestamp":1561680000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The species of<jats:italic>Anisakis<\/jats:italic>constitute one of the most widespread groups of ascaridoid nematodes in the marine ecosystem. Three closely related taxa are recognised in the<jats:italic>A. simplex<\/jats:italic>(s. l.) complex, i.e.<jats:italic>A. pegreffii<\/jats:italic>,<jats:italic>A. simplex<\/jats:italic>(s. s.) and<jats:italic>A. berlandi.<\/jats:italic>They are distributed in populations of their intermediate\/paratenic (fish and squids) and definitive (cetaceans) hosts. A panel of seven microsatellite loci (<jats:italic>Anisl 05784<\/jats:italic>,<jats:italic>Anisl 08059<\/jats:italic>,<jats:italic>Anisl 00875<\/jats:italic>,<jats:italic>Anisl 07132, Anisl 00314, Anisl 10535<\/jats:italic>and<jats:italic>Anisl 00185)<\/jats:italic>, were developed and validated on a total of<jats:italic>N<\/jats:italic>= 943 specimens of<jats:italic>A. pegreffii<\/jats:italic>and<jats:italic>A. simplex<\/jats:italic>(s. s.), collected in fish and cetacean hosts from allopatric areas within the range of distribution of these parasite species. In addition, the locus<jats:italic>Anisl 7<\/jats:italic>, previously detected in those<jats:italic>Anisakis<\/jats:italic>spp., was investigated. The parasites were first identified by sequence analysis of the EF1<jats:italic>\u03b1<\/jats:italic>-1 nDNA. The panel of the microsatellites loci here developed have allowed to: (i) detect diagnostic microsatellite loci between the two species; (ii) identify specimens of the two species<jats:italic>A. pegreffii, A. simplex<\/jats:italic>(s. s.) in a multi-marker nuclear genotyping approach; (iii) discover two sex-linked loci in both<jats:italic>Anisakis<\/jats:italic>species and (iv) estimate levels of genetic differentiation at both the inter- and intra-specific level.<\/jats:p>","DOI":"10.1017\/s003118201900074x","type":"journal-article","created":{"date-parts":[[2019,6,14]],"date-time":"2019-06-14T08:19:30Z","timestamp":1560500370000},"page":"1387-1403","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":25,"title":["Novel polymorphic microsatellite loci in<i>Anisakis pegreffii<\/i>and<i>A. simplex<\/i>(s. s.) 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