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Here we have identified and characterized a <jats:italic>\u03c3<\/jats:italic> class glycosylated GST (OoGST1), from the cattle-infective filarial nematode <jats:italic>Onchocerca ochengi,<\/jats:italic> which is homologous (99% amino acid identity) with an immunodominant GST and potential vaccine candidate from the human parasite, <jats:italic>O. volvulus<\/jats:italic>, (OvGST1b). <jats:italic>Onchocerca ochengi<\/jats:italic> native GSTs were purified using a two-step affinity chromatography approach, resolved by 2D and 1D SDS-PAGE and subjected to enzymic deglycosylation revealing the existence of at least four glycoforms. A combination of lectin-blotting and mass spectrometry (MS) analyses of the released <jats:italic>N<\/jats:italic>-glycans indicated that OoGST1 contained mainly oligomannose Man<jats:sub>5<\/jats:sub>GlcNAc<jats:sub>2<\/jats:sub> structure, but also hybrid- and larger oligommanose-type glycans in a lower proportion. Furthermore, purified OoGST1 showed prostaglandin synthase activity as confirmed by Liquid Chromatography (LC)\/MS following a coupled-enzyme assay. This is only the second reported and characterized glycosylated GST and our study highlights its potential role in host-parasite interactions and use in the study of human onchocerciasis.<\/jats:p>","DOI":"10.1017\/s0031182019000763","type":"journal-article","created":{"date-parts":[[2019,6,13]],"date-time":"2019-06-13T09:09:18Z","timestamp":1560416958000},"page":"1773-1784","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":5,"title":["Characterization of a novel glycosylated glutathione transferase of <i>Onchocerca ochengi<\/i>, closest relative of the human river blindness parasite"],"prefix":"10.1017","volume":"146","author":[{"given":"Clair","family":"Rose","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio","family":"Praulins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart D.","family":"Armstrong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aitor","family":"Casas-Sanchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jem","family":"Davis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gemma","family":"Molyneux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Yunta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zenaida","family":"Stead","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Prescott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samirah","family":"Perally","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Rutter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin L.","family":"Makepeace","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9261-7136","authenticated-orcid":false,"given":"E. 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