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Fecal samples were collected from 25 calves from birth to 12 months in the first year. In the second year, fecal samples were collected from pregnant cows (<jats:italic>n<\/jats:italic>= 29) and their calves (<jats:italic>n<\/jats:italic>= 30) from birth to 6 months. The samples were tested for<jats:italic>Cryptosporidium<\/jats:italic>and speciated.<jats:italic>Cryptosporidium parvum<\/jats:italic>-positive samples were subtyped by GP60 fragment analysis. All calves in both studies shed<jats:italic>Cryptosporidium<\/jats:italic>during the study period.<jats:italic>Cryptosporidium parvum<\/jats:italic>was the predominant species detected in calves \u2a7d6 weeks of age and at 6 months of age,<jats:italic>C. bovis<\/jats:italic>and<jats:italic>C. ryanae<\/jats:italic>were detected in calves older than 4 weeks of age but \u2a7d6 months of age. The prevalence of<jats:italic>Cryptosporidium<\/jats:italic>was higher in younger animals than in older animals. GP60 subtyping revealed two subtypes in calves on this farm (IIaA15G2R1 and IIaA19G2R1) that differed in frequency by age. Adult cattle also shed<jats:italic>C. parvum,<\/jats:italic>of four<jats:italic>gp60<\/jats:italic>genotypes.<\/jats:p>","DOI":"10.1017\/s0031182019000829","type":"journal-article","created":{"date-parts":[[2019,7,22]],"date-time":"2019-07-22T08:51:42Z","timestamp":1563785502000},"page":"1404-1413","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":44,"title":["Shedding of<i>Cryptosporidium<\/i>in calves and dams: evidence of re-infection and shedding of different<i>gp60<\/i>subtypes"],"prefix":"10.1017","volume":"146","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0549-1104","authenticated-orcid":false,"given":"Sarah","family":"Thomson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth A.","family":"Innes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas N.","family":"Jonsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Katzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2019,7,22]]},"reference":[{"key":"S0031182019000829_ref57","first-page":"88","article-title":"Public health significance of zoonotic Cryptosporidium species in wildlife: critical insights into better drinking water management","volume":"5","author":"Zahedi","year":"2016","journal-title":"International Journal for Parasitology: Parasites and Wildlife"},{"key":"S0031182019000829_ref56","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.1128\/AEM.65.4.1578-1583.1999","article-title":"Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus","volume":"65","author":"Xiao","year":"1999","journal-title":"Applied and Environmental Microbiology"},{"key":"S0031182019000829_ref61","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2008.00377.x"},{"key":"S0031182019000829_ref55","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2016.07.018"},{"key":"S0031182019000829_ref54","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-0684-x"},{"key":"S0031182019000829_ref53","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.5.2576-2579.2002"},{"key":"S0031182019000829_ref52","doi-asserted-by":"publisher","DOI":"10.1136\/vr.112.6.116"},{"key":"S0031182019000829_ref50","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-017-0447-0"},{"key":"S0031182019000829_ref45","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2014.05.022"},{"key":"S0031182019000829_ref44","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.01.003"},{"key":"S0031182019000829_ref43","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268812000830"},{"key":"S0031182019000829_ref41","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2004.03.020"},{"key":"S0031182019000829_ref39","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2013.05.001"},{"key":"S0031182019000829_ref38","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-013-3520-2"},{"key":"S0031182019000829_ref37","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.2010.00631.x"},{"key":"S0031182019000829_ref60","unstructured":"R Core Team (2016) R: A language and environment for statistical computing. 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