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In <jats:italic>Plasmodium falciparum<\/jats:italic>, another Apicomplexa protozoan, the interaction of rhoptry neck protein 2 (RON2) with apical membrane antigen-1 (AMA-1) has been described to have a key role in the invasion process. To date, RON2 has not been described in <jats:italic>Babesia bigemina<\/jats:italic>, the causal agent of bovine babesiosis in the Americas. In this work, we found a <jats:italic>ron2<\/jats:italic> gene in the <jats:italic>B. bigemina<\/jats:italic> genome. RON2 encodes a protein that is 1351 amino acids long, has an identity of 64% (98% coverage) with RON2 of <jats:italic>B. bovis<\/jats:italic> and contains the CLAG domain, a conserved domain in Apicomplexa. <jats:italic>B. bigemina ron2<\/jats:italic> is a single copy gene and it is transcribed and expressed in blood stages as determined by RT-PCR, Western blot, and confocal microscopy. Serum samples from <jats:italic>B. bigemina<\/jats:italic>-infected bovines were screened for the presence of RON2-specific antibodies, showing the recognition of conserved B-cell epitopes. Importantly, <jats:italic>in vitro<\/jats:italic> neutralization assays showed an inhibitory effect of RON2-specific antibodies on the red blood cell invasion by <jats:italic>B. bigemina<\/jats:italic>. Therefore, RON2 is a novel antigen in <jats:italic>B. bigemina<\/jats:italic> and contains conserved B-cell epitopes, which induce antibodies that inhibit merozoite invasion.<\/jats:p>","DOI":"10.1017\/s0031182019001161","type":"journal-article","created":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T07:11:47Z","timestamp":1566889907000},"page":"1646-1654","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":8,"title":["RON2, a novel gene in <i>Babesia bigemina,<\/i> contains conserved, immunodominant B-cell epitopes that induce antibodies that block merozoite invasion"],"prefix":"10.1017","volume":"146","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8892-6390","authenticated-orcid":false,"given":"Juan","family":"Mosqueda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Hidalgo-Ruiz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana Alexandra","family":"Calvo-Olvera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego Josimar","family":"Hernandez-Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massaro Wilson","family":"Ueti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel 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