{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T17:43:14Z","timestamp":1792690994376,"version":"4.5.0"},"reference-count":27,"publisher":"Cambridge University Press (CUP)","issue":"14","license":[{"start":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T00:00:00Z","timestamp":1569456000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In developing countries, estimates of the prevalence and diversity of <jats:italic>Leptospira<\/jats:italic> infections in livestock, an important but neglected zoonotic pathogen and cause of livestock productivity loss, are lacking. In Madagascar, abattoir sampling of cattle and pigs demonstrated a prevalence of infection of 20% in cattle and 5% in pigs by real-time PCR. In cattle, amplification and sequencing of the <jats:italic>Leptospira<\/jats:italic>-specific <jats:italic>lfb1<\/jats:italic> gene revealed novel genotypes, mixed infections of two or more <jats:italic>Leptospira<\/jats:italic> species and evidence for potential transmission between small mammals and cattle. Sequencing of the <jats:italic>secY<\/jats:italic> gene demonstrated genetic similarities between <jats:italic>Leptospira<\/jats:italic> detected in Madagascar and, as yet, uncultured <jats:italic>Leptospira<\/jats:italic> strains identified in Tanzania, Reunion and Brazil. Detection of <jats:italic>Leptospira<\/jats:italic> DNA in the same animal was more likely in urine samples or pooled samples from four kidney lobes relative to samples collected from a single kidney lobe, suggesting an effect of sampling method on detection. In pigs, no molecular typing of positive samples was possible. Further research into the epidemiology of livestock leptospirosis in developing countries is needed to inform efforts to reduce human infections and to improve livestock productivity.<\/jats:p>","DOI":"10.1017\/s0031182019001252","type":"journal-article","created":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T08:12:56Z","timestamp":1569485576000},"page":"1707-1713","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":16,"title":["<i>Leptospira<\/i> in livestock in Madagascar: uncultured strains, mixed infections and small mammal-livestock transmission highlight challenges in controlling and diagnosing leptospirosis in the developing world"],"prefix":"10.1017","volume":"146","author":[{"given":"Soanandrasana","family":"Rahelinirina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0807-4632","authenticated-orcid":false,"given":"Mark H.","family":"Moseley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn J.","family":"Allan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Ramanohizakandrainy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sati","family":"Ravaoarinoro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minoarisoa","family":"Rajerison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Rakotoharinome","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Telfer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2019,9,26]]},"reference":[{"key":"S0031182019001252_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjm.2017.06.008"},{"key":"S0031182019001252_ref3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0603979103"},{"key":"S0031182019001252_ref10","unstructured":"ILRI (2012) Mapping of Poverty and Likely Zoonoses Hotspots. 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