{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T23:01:20Z","timestamp":1773097280139,"version":"3.50.1"},"reference-count":41,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2019,10,23]],"date-time":"2019-10-23T00:00:00Z","timestamp":1571788800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human neurocysticercosis (NCC) is a worldwide neglected disease caused by<jats:italic>Taenia solium<\/jats:italic>metacestode and responsible for various complications and neurological disorders. This study aimed to evaluate the use of specific immunoglobulin Y (IgY) produced by laying hens immunized with a hydrophobic fraction of<jats:italic>Taenia crassiceps<\/jats:italic>metacestodes (hF<jats:italic>Tc<\/jats:italic>) in NCC diagnosis. Egg yolk IgY antibodies were fractionated, purified and characterized. Enzyme-linked immunosorbent assay (ELISA) was carried out to evaluate the production kinetics and avidity maturation of anti-hF<jats:italic>Tc<\/jats:italic>IgY antibodies throughout the IgY obtention process. Antigen recognition tests were carried out by Western blotting and immunofluorescence antibody test using purified and specific anti-hF<jats:italic>Tc<\/jats:italic>IgY antibodies for detection of parasitic antigens of<jats:italic>T. crassiceps<\/jats:italic>and<jats:italic>T. solium<\/jats:italic>metacestodes. Sandwich ELISA was performed to detect circulating immune complexes formed by IgG and parasitic antigens in human sera. The results showed high diagnostic values (93.2% sensitivity and 94.3% specificity) for immune complexes detection in human sera with confirmed NCC. In conclusion, specific IgY antibodies produced from immunized hens with hF<jats:italic>Tc<\/jats:italic>antigens were efficient to detect<jats:italic>T. solium<\/jats:italic>immune complexes in human sera, being an innovative and potential tool for NCC immunodiagnosis.<\/jats:p>","DOI":"10.1017\/s0031182019001446","type":"journal-article","created":{"date-parts":[[2019,10,11]],"date-time":"2019-10-11T09:16:56Z","timestamp":1570785416000},"page":"240-247","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":9,"title":["IgY antibody and human neurocysticercosis: a novel approach on immunodiagnosis using<i>Taenia crassiceps<\/i>hydrophobic antigens"],"prefix":"10.1017","volume":"147","author":[{"given":"Guilherme M. 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