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Serodiagnosis can be a useful tool to identify these cases, but conventional tests do not differentiate past from active infections. The aim of this study was to obtain and apply egg yolk polyclonal immunoglobulin (IgY) antibodies to detect immune complexes (ICs) in serum samples from patients infected with hookworm. Hens were immunized with<jats:italic>Ancylostoma ceylanicum<\/jats:italic>saline extract, their eggs were collected and then IgY antibodies were extracted and purified. Antibody purity was tested by 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis and specificity was assessed by immunoblotting and immunofluorescence. IgY production was evaluated by kinetics enzyme-linked immunosorbent assay (ELISA). Sandwich ELISA tested the ability of IgY to detect ICs in serum samples, from which diagnostic parameters were calculated. Antibody responses increased steadily from day 7 to 42. In the immunoblotting assay, IgY recognized two protein complexes. The immunofluorescence assay showed no staining in control samples. The sandwich ELISA presented a very high diagnostic value, with a sensitivity of 90% and a specificity of 86.7%. Our pioneer strategy highlights the potential use of egg yolk IgY as a diagnostic test to detect active hookworm infection.<\/jats:p>","DOI":"10.1017\/s0031182020000220","type":"journal-article","created":{"date-parts":[[2020,2,13]],"date-time":"2020-02-13T05:42:37Z","timestamp":1581572557000},"page":"715-720","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":7,"title":["Use of polyclonal IgY antibodies to detect serum immune complexes in patients with active hookworm infection"],"prefix":"10.1017","volume":"147","author":[{"given":"Dayane Costa de","family":"Souza","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas Silva","family":"de Faria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Eduardo Neto de","family":"Sousa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila de Almeida","family":"Lopes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa da Silva","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivian Jordania","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raphaella Paula","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c9lida Mara Leite","family":"Rabelo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veridiana Melo","family":"Rodrigues \u00c1vila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1lvaro","family":"Ferreira J\u00fanior","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5751-8511","authenticated-orcid":false,"given":"Julia Maria","family":"Costa-Cruz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2020,2,13]]},"reference":[{"key":"S0031182020000220_ref28","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12341"},{"key":"S0031182020000220_ref11","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182012000054"},{"key":"S0031182020000220_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(93)90179-B"},{"key":"S0031182020000220_ref25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0113208"},{"key":"S0031182020000220_ref24","first-page":"507","article-title":"Contribui\u00e7\u00e3o para o estudo do Strongyloides Stercoralis e da estrongiloid\u00edase no Brasil","volume":"1","author":"Moraes","year":"1948","journal-title":"Revista do Servi\u00e7o Especial de Sa\u00fade P\u00fablica"},{"key":"S0031182020000220_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2019.101933"},{"key":"S0031182020000220_ref31","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-7-37"},{"key":"S0031182020000220_ref14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0070702"},{"key":"S0031182020000220_ref33","doi-asserted-by":"publisher","DOI":"10.1177\/0192623314526475"},{"key":"S0031182020000220_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(92)90013-J"},{"key":"S0031182020000220_ref4","doi-asserted-by":"publisher","DOI":"10.1177\/1040638717706098"},{"key":"S0031182020000220_ref21","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X19000701"},{"key":"S0031182020000220_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apar.2016.08.002"},{"key":"S0031182020000220_ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2013.09.005"},{"key":"S0031182020000220_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgeb.2017.10.003"},{"key":"S0031182020000220_ref3","first-page":"41","article-title":"Eine Einfache Methode zur Auffindung von Ankylostomum (Nematoden) Larven in Erdproben. 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