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In this regard, in the present study, nanocomposite synthesized and its effects on the metabolites of <jats:italic>P. falciparum<\/jats:italic> were investigated. Subsequent to synthesis of nanocomposites, characterization was carried out using nuclear magnetic resonance (NMR), liquid chromatography-mass spectrometry (LC-MS), scanning electron microscopy, dynamic light scattering and Fourier-transform infrared tests. Solubility and drug release were measured and its toxicity on Vero cell was assessed using the MTT assay. The antiparasitic effect of the nanocomposite on the metabolites of <jats:italic>P. falciparum<\/jats:italic> was investigated by <jats:sup>1<\/jats:sup>H NMR spectroscopy. Among synthesized nanocomposites, the average size of 239 nm showed suitable solubility in water as well as slow drug release. The MTT assay showed no toxicity for Vero cell lines. Concentrations of 2.5 <jats:italic>\u03bc<\/jats:italic>g mL<jats:sup>\u22121<\/jats:sup> of nanocomposite eliminated 82.6% of the total parasites. The most effected metabolic cycles were glyoxylate and dicarboxylate metabolism. In this study, <jats:sup>1<\/jats:sup>H NMR spectroscopy was used with untargeted metabolomics to study the effect of the nanocomposite on <jats:italic>P. falciparum<\/jats:italic>. Playing an essential role in understanding drug-target interactions and characterization of mechanism of action or resistance exhibited by novel antiprotozoal drugs, can be achieved by targeting metabolic using LC-MS.<\/jats:p>","DOI":"10.1017\/s0031182020000372","type":"journal-article","created":{"date-parts":[[2020,2,27]],"date-time":"2020-02-27T09:24:41Z","timestamp":1582795481000},"page":"747-759","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":28,"title":["Novel chloroquine loaded curcumin based anionic linear globular dendrimer G2: a metabolomics study on <i>Plasmodium falciparum in vitro<\/i> using <sup>1<\/sup>H NMR spectroscopy"],"prefix":"10.1017","volume":"147","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4247-4445","authenticated-orcid":false,"given":"Taher","family":"Elmi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Shafiee 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