{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T01:44:06Z","timestamp":1785894246936,"version":"3.56.0"},"reference-count":20,"publisher":"Cambridge University Press (CUP)","issue":"7","license":[{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Cochliomyia hominivorax<\/jats:italic> larvae cause myiasis in animals and humans. To register a commercial product to control this dipteran is necessary to experiment on animals. The <jats:italic>in vitro<\/jats:italic> test was standardized to evaluate the larvicidal efficacy of commercial topical products. Five formulations were analysed <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. For the <jats:italic>in vitro<\/jats:italic> test, a colony was formed and three replicates (<jats:italic>n<\/jats:italic> = 200) of each larval stage (L1, L2 and L3) were treated. The viability of the larvae was evaluated after 5 and 30 min, and at 1, 2, 6, 12, 24, 48, 60 and 72 h post-treatment (HPT). For the <jats:italic>in vivo<\/jats:italic> test, 30 bovines divided into six groups were castrated to achieve natural infestation with <jats:italic>C. hominivorax<\/jats:italic>. Animals in the treated groups received the product. Myiasis and efficacy were evaluated 12, 24, 36, 48, 60 and 72 HPT. Four formulations tested in the <jats:italic>in vitro<\/jats:italic> test achieved 100% efficacy at 24 HPT. In the <jats:italic>in vivo<\/jats:italic> experiment only one achieved 100% efficacy at 24 HPT. However, all products achieved the maximum efficacy by the end of study. The <jats:italic>in vitro<\/jats:italic> test developed here could be adopted to evaluate the efficacy of topical products for the control of <jats:italic>C. hominivorax<\/jats:italic> larvae.<\/jats:p>","DOI":"10.1017\/s0031182020000499","type":"journal-article","created":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T06:38:17Z","timestamp":1585723097000},"page":"816-821","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":7,"title":["<i>In vitro<\/i> test for the evaluation of the efficacy of topical products for the control of <i>Cochliomyia hominivorax<\/i> larvae"],"prefix":"10.1017","volume":"147","author":[{"given":"Sabrina Nath\u00e1lia Louzada","family":"Nogueira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Fernanda","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo de Andrade","family":"Furtado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Paulino J\u00fanior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cec\u00edlia","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Mariana Alves","family":"Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo Gilberti","family":"Nascimento","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0813-3054","authenticated-orcid":false,"given":"Lorena Lopes","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vando Ed\u00e9sio","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8009-8436","authenticated-orcid":false,"given":"Welber Daniel Zanetti","family":"Lopes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Paranhos de Mendon\u00e7a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2020,4,1]]},"reference":[{"key":"S0031182020000499_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.12.035"},{"key":"S0031182020000499_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(97)00082-4"},{"key":"S0031182020000499_ref12","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-84782013001200013"},{"key":"S0031182020000499_ref10","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.14-0617"},{"key":"S0031182020000499_ref14","doi-asserted-by":"publisher","DOI":"10.1002\/9783527802883.ch9"},{"key":"S0031182020000499_ref19","unstructured":"SINDAN (2018) Mercado. In: Sindicado Nacional Da Ind\u00fastria De Produtos Para Sa\u00fade Animal. Available at http:\/\/www.sindan.org.br\/sd\/base.aspx?controle=8"},{"key":"S0031182020000499_ref18","first-page":"1","article-title":"Endectocide activity of a pour-on formulation containing 1.5 per centivermectin + 0.5 per cent abamectinin cattle","volume":"16","author":"Silva","year":"2015","journal-title":"The Veterinary Record Open"},{"key":"S0031182020000499_ref20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2885.2012.01380.x"},{"key":"S0031182020000499_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2014.01.012"},{"key":"S0031182020000499_ref17","first-page":"14","article-title":"Factors of susceptibility of human myiasis caused by the New World screw-worm, Cochliomyia hominivorax in S\u00e3o Gon\u00e7alo, Rio de Janeiro, Brazil","volume":"11","author":"Silva","year":"2011","journal-title":"Journal of Insect Science"},{"key":"S0031182020000499_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.12.017"},{"key":"S0031182020000499_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2014.11.001"},{"key":"S0031182020000499_ref5","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-736X2016000800006"},{"key":"S0031182020000499_ref11","first-page":"33","article-title":"Avalia\u00e7\u00e3o da efic\u00e1cia preventiva da doramectina 3,5%* contra larvas de Cochliomyia hominivorax (mi\u00edases) em bolsas escrotais de bovinos rec\u00e9m-castrados","volume":"29","author":"Lopes","year":"2009","journal-title":"A Hora Veterin\u00e1ria"},{"key":"S0031182020000499_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2006.07.001"},{"key":"S0031182020000499_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.09.018"},{"key":"S0031182020000499_ref1","unstructured":"BRASIL. Portaria No 48, de 12 de Maio de 1997, do minist\u00e9rio da Agricultura e do Abastecimento. Regulamento T\u00e9cnico para Licenciamento e\/ou Renova\u00e7\u00e3o de Licen\u00e7a de Produtos Antiparasit\u00e1rios de Uso Veterin\u00e1rio. Dispon\u00edvel em. Available at http:\/\/sistemasweb.agricultura.gov.br\/sislegis\/action\/detalhaAto.do?method=visualizarAtoPortalMapa&chave=72818869"},{"key":"S0031182020000499_ref6","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1590\/S1984-29612014042","article-title":"Reassessment of the potential economic impact of cattle parasites in Brazil","volume":"23","author":"Grisi","year":"2014","journal-title":"Revista Brasileira de Parasitologia Veterin\u00e1ria"},{"key":"S0031182020000499_ref15","unstructured":"Oliveira, MCS , Brito, LG , Giglioti, R and Chagas, ACS (2009) Manuten\u00e7\u00e3o de culturas in vitro da mosca da bicheira, Cochliomyia hominivorax. Comunicado T\u00e9cnico 339, Embrapa Rond\u00f4nia, Porto Velho."},{"key":"S0031182020000499_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2005.11.006"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182020000499","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T21:30:10Z","timestamp":1611869410000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182020000499\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,1]]},"references-count":20,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["S0031182020000499"],"URL":"https:\/\/doi.org\/10.1017\/s0031182020000499","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,1]]},"assertion":[{"value":"Copyright \u00a9 The Author(s), 2020. 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