{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T22:49:40Z","timestamp":1785797380180,"version":"3.56.0"},"reference-count":58,"publisher":"Cambridge University Press (CUP)","issue":"10","license":[{"start":{"date-parts":[[2020,6,2]],"date-time":"2020-06-02T00:00:00Z","timestamp":1591056000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Due to the efforts to control schistosomiasis transmission in tropical countries, a large proportion of individuals from endemic areas present low parasite loads, which hinders diagnosis of intestinal schistosomiasis by the Kato-Katz (KK) method. Therefore, the development of more sensitive diagnostic methods is essential for efficient control measures. The aim was to evaluate the accuracy of a real-time polymerase chain reaction (RT-PCR) to detect <jats:italic>Schistosoma mansoni<\/jats:italic> DNA in fecal samples of individuals with low parasite loads. A cross-sectional population-based study was conducted in a rural community (<jats:italic>n<\/jats:italic> = 257) in Brazil. POC-CCA<jats:sup>\u00ae<\/jats:sup> was performed in urine and feces were used for RT-PCR. In addition, fecal exams were completed by 18 KK slides, saline gradient and Helmintex techniques. The combined results of the three parasitological tests detected schistosome eggs in 118 participants (45.9%) and composed the consolidated reference standard (CRS). By RT-PCR, 117 out of 215 tested samples were positive, showing 91.4% sensitivity, 80.2% specificity and good concordance with the CRS (kappa = 0.71). RT-PCR identified 86.9% of the individuals eliminating less than 12 eggs\/g of feces, demonstrating much better performance than POC-CCA<jats:sup>\u00ae<\/jats:sup> (50.8%). Our results showed that RT-PCR is a valuable alternative for the diagnosis of intestinal schistosomiasis in individuals with very low parasite loads.<\/jats:p>","DOI":"10.1017\/s003118202000089x","type":"journal-article","created":{"date-parts":[[2020,6,2]],"date-time":"2020-06-02T09:44:57Z","timestamp":1591091097000},"page":"1140-1148","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":34,"title":["Accuracy of real-time polymerase chain reaction to detect <i>Schistosoma mansoni \u2013<\/i> infected individuals from an endemic area with low parasite loads"],"prefix":"10.1017","volume":"147","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7928-8294","authenticated-orcid":false,"given":"Fernanda do Carmo","family":"Magalh\u00e3es","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samira 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