{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T18:19:35Z","timestamp":1757701175586,"version":"3.40.5"},"reference-count":44,"publisher":"Cambridge University Press (CUP)","issue":"13","license":[{"start":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T00:00:00Z","timestamp":1596067200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, we evaluated the efficacy, expressed as a mean weight decrease of the whole echinococcal cyst mass, of novel benzimidazole salt formulations in a murine<jats:italic>Echinococcus granulosus<\/jats:italic>infection model. BALB\/c mice were intraperitoneally infected with protoscoleces of<jats:italic>E. granulosus<\/jats:italic>(genotype G1). At 9 months post-infection, treatment with albendazole (ABZ), ricobendazole (RBZ) salt formulations, and RBZ enantiomer salts (<jats:italic>R<\/jats:italic>)-(+)-RBZ-Na and (<jats:italic>S<\/jats:italic>)-(\u2212)-RBZ-Na formulations were initiated. Drugs were orally applied by gavage at 10 mg kg<jats:sup>\u22121<\/jats:sup>body weight per day during 30 days. Experimental treatments with benzimidazole sodium salts resulted in a significant reduction of the weight of cysts compared to conventional ABZ treatment, except for the (<jats:italic>S<\/jats:italic>)-(\u2212)-RBZ-Na enantiomer formulation. Scanning electron microscopy and histological inspection revealed that treatments impacted not only the structural integrity of the parasite tissue in the germinal layer, but also induced alterations in the laminated layer. Overall, these results demonstrate the improved efficacy of benzimidazole salt formulations compared to conventional ABZ treatment in experimental murine cystic echinococcosis.<\/jats:p>","DOI":"10.1017\/s0031182020001225","type":"journal-article","created":{"date-parts":[[2020,7,30]],"date-time":"2020-07-30T10:32:24Z","timestamp":1596105144000},"page":"1425-1432","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":7,"title":["Efficacy of novel albendazole salt formulations against secondary cystic echinococcosis in experimentally infected mice"],"prefix":"10.1017","volume":"147","author":[{"given":"G.","family":"Vural","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Yardimci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kocak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. \u00d6.","family":"Yasar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Kurt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. S.","family":"Harem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Carradori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Sciamanna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Siles-Lucas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Fabiani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0622-2128","authenticated-orcid":false,"given":"A.","family":"Hemphill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2672-5766","authenticated-orcid":false,"given":"B.","family":"Lundstr\u00f6m-Stadelmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Cirilli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9183-7591","authenticated-orcid":false,"given":"A.","family":"Casulli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2020,7,30]]},"reference":[{"key":"S0031182020001225_ref29","doi-asserted-by":"crossref","first-page":"794","DOI":"10.5962\/bhl.title.5868","volume-title":"The Microtomist's Formulary and Guide","author":"Gray","year":"1954"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref25","DOI":"10.1016\/S0378-4347(98)00497-6"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref10","DOI":"10.1016\/S1090-0233(02)00158-2"},{"key":"S0031182020001225_ref40","first-page":"42","article-title":"Efficacy of nocodazole et experimental invasion Echinococcus granulosus of white mice","volume":"2","author":"Shkoliar","year":"2014","journal-title":"Medical Parazitology (Mosk)"},{"key":"S0031182020001225_ref39","first-page":"97","article-title":"Methacarn (methanol-Carnoy) fixation. Practical and theoretical considerations","volume":"21","author":"Puchtler","year":"1970","journal-title":"Histochemie, Histochemistry, Histochimie"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref23","DOI":"10.1016\/S0378-5173(02)00559-8"},{"key":"S0031182020001225_ref32","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0001-706X(96)00640-7","article-title":"Albendazole in treatment of human cystic echinococcosis: 12 years of experience","volume":"64","author":"Horton","year":"1997","journal-title":"Acta Tropica"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref24","DOI":"10.1007\/s004360050219"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref17","DOI":"10.1016\/j.jpba.2017.01.057"},{"key":"S0031182020001225_ref6","first-page":"459","article-title":"In vitro observation on albendazole sulfoxide and its enantiomers against Echinococcus granulosus protoscolex","volume":"26","author":"Bao","year":"2008","journal-title":"Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi"},{"key":"S0031182020001225_ref5","first-page":"112","volume-title":"Theory and Practice of Histological Techniques","author":"Bancroft","year":"1990"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref38","DOI":"10.1371\/journal.pntd.0005114"},{"key":"S0031182020001225_ref31","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1017\/S0022149X15000516","article-title":"Molecular genotyping of Echinococcus granulosus using formalin-fixed paraffin-embedded preparations from human isolates in unusual tissue sites","volume":"90","author":"Hizem","year":"2016","journal-title":"Journal of Helminthology"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref1","DOI":"10.1128\/JCM.02420-15"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref43","DOI":"10.1016\/S1473-3099(18)30221-4"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref33","DOI":"10.1016\/bs.apar.2016.09.006"},{"unstructured":"Cirilli, R , Carradori, S , Casulli, A and De Monte, C (2020) WO2017021992 \u2013 Salts of compounds having a benzimidazolic structure, uses and process for the preparation thereof. Available at https:\/\/patentscope.wipo.int\/search\/en\/detail.jsf?docId=WO2017021992 (Accessed 15 June 2020).","key":"S0031182020001225_ref18"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref30","DOI":"10.1007\/978-3-642-68029-8"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref12","DOI":"10.1016\/S2214-109X(20)30066-8"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref28","DOI":"10.1080\/14787210.2018.1555241"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref11","DOI":"10.1007\/BF00931155"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref42","DOI":"10.1016\/j.ijpara.2004.01.006"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref2","DOI":"10.1016\/S0304-4017(00)00320-4"},{"volume-title":"Modern Electron Microscopy in Physical and Life Sciences","year":"2016","author":"Murtey","key":"S0031182020001225_ref35"},{"unstructured":"Patentscope database (2020) Available at https:\/\/www.wipo.int\/patentscope\/en\/ (Accessed 15 June 2020).","key":"S0031182020001225_ref37"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref4","DOI":"10.1007\/s00436-019-06416-5"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref41","DOI":"10.1371\/journal.pntd.0006422"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref34","DOI":"10.1097\/MD.0000000000002237"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref19","DOI":"10.1016\/j.vetpar.2012.11.027"},{"key":"S0031182020001225_ref7","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1645\/GE-3212RN","article-title":"Ex vivo anthelmintic activity of albendazole-sulphoxide enantiomers","volume":"90","author":"Bol\u00e1s-Fern\u00e1ndez","year":"2004","journal-title":"Journal of Parasitology"},{"key":"S0031182020001225_ref16","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.actatropica.2015.04.019","article-title":"Combined flubendazole-nitazoxanide treatment of cystic echinococcosis: pharmacokinetic and efficacy assessment in mice","volume":"148","author":"Ceballos","year":"2015","journal-title":"Acta Tropica"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref8","DOI":"10.1016\/j.actatropica.2009.11.001"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref14","DOI":"10.1016\/j.pt.2019.10.009"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref44","DOI":"10.1016\/j.vetpar.2008.03.020"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref13","DOI":"10.1016\/j.pt.2019.05.006"},{"key":"S0031182020001225_ref36","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1128\/AAC.01465-12","article-title":"In vitro analysis of albendazole sulfoxide enantiomers shows that (+)-(R)-albendazole sulfoxide is the active enantiomer against Taenia solium","volume":"57","author":"Paredes","year":"2013","journal-title":"Antimicrobial Agents and Chemotherapy"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref21","DOI":"10.3109\/00498259109039463"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref27","DOI":"10.1007\/s002689910115"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref22","DOI":"10.1007\/s00436-006-0381-y"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref3","DOI":"10.1016\/j.ijsu.2008.08.003"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref9","DOI":"10.1371\/journal.pntd.0001146"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref15","DOI":"10.1016\/j.actatropica.2013.05.004"},{"doi-asserted-by":"publisher","key":"S0031182020001225_ref26","DOI":"10.4269\/ajtmh.2009.80.367"},{"key":"S0031182020001225_ref20","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1002\/chir.530020306","article-title":"Species differences in the generation of the chiral sulfoxide metabolite of albendazole in sheep and rats","volume":"2","author":"Delatour","year":"1990","journal-title":"Chirality"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182020001225","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,5]],"date-time":"2022-11-05T04:25:03Z","timestamp":1667622303000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182020001225\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,30]]},"references-count":44,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2020,11]]}},"alternative-id":["S0031182020001225"],"URL":"https:\/\/doi.org\/10.1017\/s0031182020001225","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"type":"print","value":"0031-1820"},{"type":"electronic","value":"1469-8161"}],"subject":[],"published":{"date-parts":[[2020,7,30]]},"assertion":[{"value":"Copyright \u00a9 The Author(s), 2020. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}