{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T20:46:34Z","timestamp":1786999594766,"version":"build-2736575974"},"reference-count":35,"publisher":"Cambridge University Press (CUP)","issue":"14","license":[{"start":{"date-parts":[[2020,10,2]],"date-time":"2020-10-02T00:00:00Z","timestamp":1601596800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    VmCT1 is an antimicrobial peptide (AMP) isolated from the venom of the scorpion\n                    <jats:italic>Vaejovis mexicanus<\/jats:italic>\n                    with antimicrobial, anticancer and antimalarial activities, which the rational design with Arg-substitution has yielded AMPs with higher antimicrobial activity than VmCT1. Chagas is a neglected tropical disease, becoming the development of new antichagasic agents is urgent. Thus, we aimed to evaluate the antichagasic effect of VmCT1 and three Arg-substituted analogues, as well their action mechanism. Peptides were tested against the epimastigote, trypomastigote, amastigote forms of\n                    <jats:italic>Trypanossoma cruzi<\/jats:italic>\n                    Y strain and against LLC-MK2 mammalian cells. The mechanism of action of these peptides was evaluated by means of flow cytometry and scanning electron microscopy. VmCT1 presented activity against all three forms of\n                    <jats:italic>T. cruzi<\/jats:italic>\n                    , with EC\n                    <jats:sub>50<\/jats:sub>\n                    against trypomastigote forms of 1.37\n                    <jats:italic>\u03bc<\/jats:italic>\n                    mol L\n                    <jats:sup>\u22121<\/jats:sup>\n                    and selectivity index (SI) of 58. [Arg]\n                    <jats:sup>3<\/jats:sup>\n                    -VmCT1, [Arg]\n                    <jats:sup>7<\/jats:sup>\n                    -VmCT1 and [Arg]\n                    <jats:sup>11<\/jats:sup>\n                    -VmCT1 also showed trypanocidal effect, but [Arg]\n                    <jats:sup>11<\/jats:sup>\n                    -VmCT1 had the best effect, being able to decrease the EC\n                    <jats:sub>50<\/jats:sub>\n                    against trypomastigote forms to 0.8\n                    <jats:italic>\u03bc<\/jats:italic>\n                    mol L\n                    <jats:sup>\u22121<\/jats:sup>\n                    and increase SI to 175. Necrosis was cell death pathway of VmCT1, as well [Arg]\n                    <jats:sup>7<\/jats:sup>\n                    -VmCT1 and [Arg]\n                    <jats:sup>11<\/jats:sup>\n                    -VmCT1, such as observed by membrane damage in flow cytometry analyses and scanning-electron-microscopy. In conclusion, [Arg]\n                    <jats:sup>11<\/jats:sup>\n                    -VmCT1 revealed promising as a candidate for new antichagasic therapeutics.\n                  <\/jats:p>","DOI":"10.1017\/s0031182020001882","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T23:30:29Z","timestamp":1601595029000},"page":"1810-1818","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":15,"title":["Arg-substituted VmCT1 analogs reveals promising candidate for the development of new antichagasic agent"],"prefix":"10.1017","volume":"147","author":[{"given":"Cibele Nicolaski","family":"Pedron","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katielle Albuquerque","family":"Freire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo Der Torossian","family":"Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00e2nya Bandeira","family":"Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00edlia Lopes","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramon R\u00f3seo Paula Pessoa Bezerra de","family":"Menezes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8160-2027","authenticated-orcid":false,"given":"Alice Maria Costa","family":"Martins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9420-0956","authenticated-orcid":false,"suffix":"Junior","given":"Vani Xavier","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2020,10,2]]},"reference":[{"key":"S0031182020001882_ref30","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-018-0224-2"},{"key":"S0031182020001882_ref18","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1370-2"},{"key":"S0031182020001882_ref4","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182017001846"},{"key":"S0031182020001882_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2012.02.002"},{"key":"S0031182020001882_ref15","doi-asserted-by":"publisher","DOI":"10.2174\/1568026616666160713122404"},{"key":"S0031182020001882_ref10","doi-asserted-by":"publisher","DOI":"10.4155\/fmc-2018-0460"},{"key":"S0031182020001882_ref27","doi-asserted-by":"publisher","DOI":"10.3390\/toxins11100563"},{"key":"S0031182020001882_ref11","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00091"},{"key":"S0031182020001882_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2016.11.040"},{"key":"S0031182020001882_ref12","first-page":"166","article-title":"Chagas 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Disease Reference Group on Chagas Disease, Human African Trypanosomiasis and Leishmaniasis: WHO Technical Report Series (No. 975). Research Priorities for Chagas Disease, Human African Trypanosomiasis and Leishmaniasis Research Priorities for Chagas Disea. doi: ISSN 0512-3054."}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182020001882","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T04:01:40Z","timestamp":1606795300000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182020001882\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,2]]},"references-count":35,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["S0031182020001882"],"URL":"https:\/\/doi.org\/10.1017\/s0031182020001882","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,2]]},"assertion":[{"value":"Copyright \u00a9 The Author(s) 2020. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}