{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T17:11:26Z","timestamp":1791825086042,"version":"4.3.4"},"reference-count":25,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2020,10,2]],"date-time":"2020-10-02T00:00:00Z","timestamp":1601596800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Serological tests may yield false-negative results for specific antibodies detection before or at the early seroconversion phase. Tests that detect circulating antigens of <jats:italic>Angiostrongylus cantonensis<\/jats:italic> would therefore be of value in diagnosis to distinguish current or past infection. Here, a quick, easy to perform, portable and inexpensive diagnostic device for detection of 31-kDa <jats:italic>A. cantonensis<\/jats:italic> specific antigens had been developed. This sandwich dot-immunogold filtration assay (<jats:italic>Ac<\/jats:italic>DIGFA<jats:sup>Ag<\/jats:sup>), for detecting active angiostrongyliasis was produced using anti-<jats:italic>A. cantonensis<\/jats:italic> polyclonal antibody dotted on the nitrocellulose membrane as a capture agent and colloidal gold-labelled anti-31 kDa <jats:italic>A. cantonensis<\/jats:italic> antibody as a detection agent. A well-defined pink dot, indicating positivity, was seen readily by naked eye within 10\u201315 min. The <jats:italic>Ac<\/jats:italic>DIGFA<jats:sup>Ag<\/jats:sup> detected <jats:italic>A<\/jats:italic>. <jats:italic>cantonensis<\/jats:italic>-specific antigens in cerebrospinal fluid samples from 4 out of 10 serologically confirmed angiostrongyliasis cases and 2 out of 5 suspected cases with negative anti-<jats:italic>A<\/jats:italic>. <jats:italic>cantonensis<\/jats:italic> antibodies. Among the 19 patient sera with <jats:italic>A. cantonensis<\/jats:italic> infection, 2 showed positive reaction by <jats:italic>Ac<\/jats:italic>DIGFA<jats:sup>Ag<\/jats:sup>. No positive <jats:italic>Ac<\/jats:italic>DIGFA<jats:sup>Ag<\/jats:sup> reaction was observed in all the serum samples with other parasitic diseases, and the healthy controls. The present \u2018<jats:italic>Ac<\/jats:italic>DIGFA<jats:sup>Ag<\/jats:sup>\u2019 enables rapid qualitative detection of the specific 31-kDa antigens of <jats:italic>A. cantonensis<\/jats:italic> in clinical samples with potential for application even under resource-limited settings.<\/jats:p>","DOI":"10.1017\/s0031182020001894","type":"journal-article","created":{"date-parts":[[2020,10,2]],"date-time":"2020-10-02T03:30:45Z","timestamp":1601609445000},"page":"234-239","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":5,"title":["Sandwich dot-immunogold filtration assay (DIGFA) for specific immunodiagnosis of active neuroangiostrongyliasis"],"prefix":"10.1017","volume":"148","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5764-5526","authenticated-orcid":false,"given":"Praphathip","family":"Eamsobhana","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anchalee","family":"Tungtrongchitr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoi-Sen","family":"Yong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anchana","family":"Prasartvit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darawan","family":"Wanachiwanawin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Xian","family":"Gan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2020,10,2]]},"reference":[{"key":"S0031182020001894_ref18","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201900244"},{"key":"S0031182020001894_ref25","first-page":"55","article-title":"The current status of laboratory diagnosis of Angiostrongylus cantonensis infections in humans using serologic and molecular methods","volume":"72","author":"Wilkins","year":"2013","journal-title":"Hawai'i Journal of Medicine and Public Health"},{"key":"S0031182020001894_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(91)90007-T"},{"key":"S0031182020001894_ref13","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762013000100020"},{"key":"S0031182020001894_ref2","doi-asserted-by":"publisher","DOI":"10.1645\/GE-2590.1"},{"key":"S0031182020001894_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2008.09.021"},{"key":"S0031182020001894_ref15","first-page":"121","article-title":"Evaluation of dot immunogold filtration assay (DIGFA) for rapid serodiagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea)","volume":"32","author":"Eamsobhana","year":"2015","journal-title":"Tropical Biomedicine"},{"key":"S0031182020001894_ref17","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00044-08"},{"key":"S0031182020001894_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70229-9"},{"key":"S0031182020001894_ref12","first-page":"308","article-title":"Purification of a specific immunodiagnostic Parastrongylus cantonensis antigen by electroelution from SDS-polyacrylamide gels","volume":"32","author":"Eamsobhana","year":"2001","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0031182020001894_ref4","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1997.56.408"},{"key":"S0031182020001894_ref6","unstructured":"Eamsobhana, P (1994) Immunological studies on the rat lung-worm Angiostrongylus cantonensis (Nematoda: Metastrongylidae) . 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